An evolutionarily conserved G-protein coupled receptor family, SREB, expressed in the central nervous system
An evolutionarily conserved G-protein coupled receptor family, SREB, expressed in the central nervous system
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DOI：
10.1006/bbrc.2000.2829
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发表时间：
2000-06-07
影响因子：
3.1
通讯作者：
Furuichi, K
中科院分区：
文献类型：
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作者：
Matsumoto, M;Saito, T;Furuichi, K
我们在这里报告了一个新的家庭的G蛋白偶联受体（GPCR），这是非常保守的脊椎动物物种。这个家族被命名为SREB（脑中表达的超保守受体），由至少三个成员组成，称为SREB1，SREB2和SREB3。SREB成员彼此共有52 - 63%的氨基酸同一性，并且与先前已知的胺GPCR（约25%同一性）显示出相对高的相似性。人和大鼠直系同源物之间的氨基酸序列同一性对于SREB1为97%，对于SREB3为99%，而SREB2序列在物种之间令人惊讶地完全相同。此外，斑马鱼SREB2和SREB3的氨基酸序列与哺乳动物直系同源物的同源性分别为94%和78%。北方印迹分析显示，SREB成员主要在脑区域和生殖器官中表达。辐射杂交分析将SREB1、SREB2和SREB3基因分别定位于不同的人类染色体，即3p21-p14、7q31和Xp11。在中枢神经系统中的高序列保守性和丰富表达表明存在由SREB家族成员及其内源性配体组成的未发现的基础神经元系统。(C)北京大学出版社.
We report here a novel family of G-protein coupled receptor (GPCR) which is extraordinarily conserved among vertebrate species. This family, designated SREB (Super Conserved Receptor Expressed in Brain), consists of at least three members, termed SREB1, SREB2, and SREB3. SREB members share 52-63% amino acid identity with each other and show relatively high similarity to previously known amine amine GPCRs (approximately 25% identity). Amino acid sequence identity between human and rat orthologues is 97% for SREB1 and 99% for SREB3, while the SREB2 sequence is surprisingly completely identical between the species. Furthermore, amino acid sequence of zebrafish SREB2 and SREB3 are 94 and 78% identical to mammal orthologues. Northern blot analysis revealed that SREB members are predominantly expressed in the brain regions and genital organs. Radiation hybrid analysis localized SREB1, SREB2, and SREB3 genes to different human chromosomes, namely 3p21-p14, 7q31 and Xp11, respectively. The high sequence conservation and abundant expression in the central nervous system suggest the existence of undiscovered fundamental neuronal systems consisting of SREB family members and their endogenous ligand(s). (C) 2000 Academic Press.