Selective chemical intervention in the proteome of Caenorhabditis elegans.
Selective chemical intervention in the proteome of Caenorhabditis elegans.
复制标题
对秀丽隐杆线虫蛋白质组的选择性化学干预。
DOI：
10.1021/pr100427c
复制
发表时间：
2010
影响因子：
4.4
通讯作者：
Husi H
中科院分区：
文献类型：
--
作者：
Husi H
我们首次研究了秀丽隐杆线虫中配体对蛋白质的调节。配体为亲环素的肽基-脯氨基异构酶抑制剂。观察到几种热休克蛋白的上调，特别是一个配体导致亲环素Cyn-5的2倍以上的增强。此外，几种代谢酶的水平也有所升高。这种方法使用了无标记的相对定量，提供了一种非常有吸引力的方法来测量配体对整个蛋白质组的影响，只需最少的样品前处理，这可能适用于大规模的研究。在这项初步研究中，比较了三种配体的作用，已经鉴定出54种独特的蛋白质，它们在给定的配体存在的情况下上调(51)或下调(3)。总共431C。鉴定了ELEX转运蛋白。我们的方法为在整个生物体水平上筛选新的和未经测试的配体的作用提供了一个耐人寻味的新方向。
We present the first study of protein regulation by ligands inCaenorhabditis elegans. The ligands were peptidyl-prolyl isomerase inhibitors of cyclophilins. Up-regulation is observed for several heat shock proteins and one ligand in particular caused a greater than 2-fold enhancement of cyclophilin CYN-5. Additionally, several metabolic enzymes display elevated levels. This approach, using label-free relative quantification, provides an extremely attractive way of measuring the effect of ligands on an entire proteome, with minimal sample pretreatment, which could be applicable to large-scale studies. In this initial study, which compares the effect of three ligands, 54 unique proteins have been identified that are up- (51) or down- (3) regulated in the presence of a given ligand. A total of 431C. elegansproteins were identified. Our methodology provides an intriguing new direction forin vivoscreening of the effects of novel and untested ligands at the whole organism level.