Mu opioid receptors are in discrete hippocampal interneuron subpopulations
Mu opioid receptors are in discrete hippocampal interneuron subpopulations
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DOI：
10.1002/hipo.1107
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发表时间：
2002-01-01
期刊：
影响因子：
3.5
通讯作者：
Milner, TA
中科院分区：
文献类型：
--
作者：
Drake, CT;Milner, TA
在大鼠海马形成中，应用mu阿片受体（MOR）激动剂解除对主要细胞的抑制，促进兴奋依赖性过程，如癫痫发生和长期增强。然而，MORs在特定抑制回路中的精确位置尚未确定，并且MORs在内源性功能中的作用尚不清楚。为了解决这些问题，使用光镜和电子显微镜检查了在CA1区域的几个抑制性海马神经元群中morr样免疫反应性（40）的分布。我们发现，在许多含有细小蛋白的篮状细胞中都存在mir -li，但在胆囊收缩素标记的篮状细胞中不存在。mori -li也普遍存在于含有生长抑素-li或神经肽Y-li的中间神经元中，类似于支配锥体细胞远端树突的“取向-空洞-分子”（O-LM）中间神经元。最后，mori -li存在于一些血管活性肠肽或含有钙黄蛋白的谱中，类似于主要支配其他中间神经元的中间神经元。这些发现表明，含有莫尔的神经元形成了海马gaba能神经元的一个神经化学和功能异质性的亚群。MORs最常发生在专门抑制锥体细胞的中间神经元上，也发生在有限数量的靶向其他中间神经元的中间神经元上。此外，MORs分布在不同类型的神经层中，其中一些相对远离内源性阿片类药物，表明其正常功能作用不同于外源性激动剂（如吗啡）的作用。(C) 2002 Wiley-Liss, Inc。
In the rat hippocampal formation, application of mu opioid receptor (MOR) agonists disinhibits principal cells, promoting excitation-dependent processes such as epileptogenesis and long-term potentiation. However, the precise location of MORs in particular inhibitory circuits, has not been determined, and the roles of MORs in endogenous functioning are unclear. To address these issues, the distribution of MOR-like immunoreactivity (40 was examined in several populations of inhibitory hippocampal neurons in the CA1 region using light and electron microscopy. We found that MOR-li was present in many parvalbumin-containing basket cells, but absent from cholecystokinin-labeled basket cells. MOR-li was also commonly in interneurons containing somatostatin-li or neuropeptide Y-li that resembled the "oriens-lacunosum-moleculare" (O-LM) interneurons innervating pyramidal cell distal dendrites. Finally, MOR-li was in some vasoactive intestinal peptide- or calretinin-containing profiles resembling interneurons that primarily innervate other interneurons. These findings indicate that MOR-containing neurons form a neurochemically and functionally heterogeneous subset of hippocampal GABAergic neurons. MORs are most frequently on interneurons that are specialized to inhibit pyramidal cells, and are on a limited number of interneurons that target other interneurons. Moreover, the distribution of MORs to different neuronal types in several laminae, some relatively far from endogenous opioids, suggests normal functional roles that are different from the actions seen with exogenous agonists such as morphine. (C) 2002 Wiley-Liss, Inc.