ESTRADIOL INDUCED PROTEINS IN THE MCF7 HUMAN-BREAST CANCER CELL-LINE
ESTRADIOL INDUCED PROTEINS IN THE MCF7 HUMAN-BREAST CANCER CELL-LINE
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DOI：
10.1016/0006-291x(79)91250-6
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发表时间：
1979-01-01
影响因子：
3.1
通讯作者：
ROCHEFORT, H
中科院分区：
文献类型：
--
作者：
WESTLEY, B;ROCHEFORT, H
用类固醇培养MCF7细胞，用(35S)-蛋氨酸标记，用一维和二维凝胶电泳检测分泌蛋白和细胞内蛋白。雌二醇（0.1 nM）增加了部分分泌蛋白的合成；诱导一个分子量为4.6万道尔顿的蛋白质是最引人注目的。46000道尔顿蛋白在分子量和等电点上具有异质性。抗雌激素的他莫昔芬不刺激任何雌激素诱导蛋白的合成，但完全抑制雌二醇的诱导。雌二醇对体内蛋白质的影响要微妙得多；只有三个。apprx。250种蛋白质受到刺激。这些蛋白质的功能尚不清楚。它们可能是研究雌激素和抗雌激素在乳腺癌中的作用机制的良好标志物，并可能与雌激素对细胞增殖的控制有关。
MCF7 cells were cultured with steroids, labeled with (35S)-methionine and the secreted and intracellular proteins were examined by 1 and 2 dimensional gel electrophoresis. Estradiol (0.1 nM) increased the synthesis of some of the secreted proteins; the induction of a protein of MW 46,000 daltons being the most dramatic. The 46,000 daltons protein was heterogeneous regarding MW and isoelectric point. The antiestrogen tamoxifen did not stimulate the synthesis of any of the estrogen induced proteins, but completely inhibited the induction by estradiol. The effect of estradiol on internal proteins was much more subtle; only 3 of .apprx. 250 proteins were stimulated. The functions of these proteins are unknown. They may be good markers for studying the mechanism of action of estrogens and antiestrogens in breast cancer, and may be related to the control of cell proliferation by estrogen.