Quinolone resistance of Salmonella enterica serovar virchow isolates from humans and poultry in Israel:: Evidence for clonal expansion
Quinolone resistance of Salmonella enterica serovar virchow isolates from humans and poultry in Israel:: Evidence for clonal expansion
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DOI：
10.1128/jcm.00062-07
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发表时间：
2007-08-01
影响因子：
9.4
通讯作者：
Yaron, Sima
中科院分区：
文献类型：
--
作者：
Solnik-Isaac, Hadas;Weinberger, Miriam;Yaron, Sima
肠道沙门氏菌在以色列的人和农场动物中高度流行。除了对多种抗生素的高耐药率外，该血清型对奈利地酸的耐药率也很高。从1997年至2004年从人和禽源分离的90%以上的沙门氏菌对奈利地酸(MIC2：128mU g/ml)耐药，对环丙沙星的敏感性降低(MIC值在0.125~0.250 mU g/m l之间)。大多数分离物属于两种优势的、密切相关的脉冲场凝胶电泳图类型。对喹诺酮类耐药机制的研究表明，该病原菌可能来自一个亲本克隆，该克隆过度生产AcrAB外排泵，并在gyrA发生单点突变，导致Asp87Tyr替换。人和家禽分离株之间的密切相似性表明，家禽可能是食物链中的Virchow沙门氏菌的来源。
Salmonella enterica serovar Virchow is highly prevalent in humans and farm animals in Israel. In addition to high rates of resistance to multiple antibiotics, this serovar exhibits a high incidence of resistance to nalidixic acid. More than 90% of Salmonella serovar Virchow isolates of human and poultry origin obtained from 1997 to 2004 were resistant to nalidixic acid (MIC 2: 128 mu g/ml), with reduced susceptibility to ciprofloxacin (MIC between 0.125 and 0.250 mu g/ml). Most isolates belonged to two predominant, closely related pulsed-field gel electrophoresis image types. Investigation of the mechanisms of quinolone resistance revealed that this pathogen probably emerged from a parental clone that overproduced the AcrAB efflux pump and had a single point mutation in gyrA leading to the Asp87Tyr substitution. The close resemblance between human and poultry isolates points to poultry as a likely source of Salmonella serovar Virchow in the food chain.