The apical ectodermal ridge regulates Hox-7 and Hox-8 gene expression in developing chick limb buds.
The apical ectodermal ridge regulates Hox-7 and Hox-8 gene expression in developing chick limb buds.
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DOI：
10.1101/gad.5.12b.2363
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发表时间：
1991-12
影响因子：
10.5
通讯作者：
B. Robert;G. Lyons;B. Simandl;A. Kuroiwa;M. Buckingham
中科院分区：
文献类型：
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作者：
B. Robert;G. Lyons;B. Simandl;A. Kuroiwa;M. Buckingham
我们发现，表达的两个相关的鸡同源盒基因，Hox-7和Hox-8，这是广泛分布在外侧中胚层在早期阶段，成为限制下的顶端外胚层嵴四肢发展的中胚层。在肢芽中胚层中的表达不维持在无肢突变体中，其不形成顶脊。突变体可以通过将正常的外胚层移植到肢体区域来拯救。这导致两个同源盒基因在诱导脊下的中胚层中表达。在肢芽上形成异位脊的真倍足的表型在两个脊下表达两个基因。当鹌鹑岭是嫁接在nonexpressing中胚层，一个新的网站的表达诱导。因此，Hox-7和Hox-8在肢芽中的持续表达依赖于功能脊，并且可以由其诱导。
We show that expression of the two related chicken homeo box genes, Hox-7 and Hox-8, which is widespread in the lateral mesoderm at early stages, becomes restricted to the mesoderm underlying the apical ectodermal ridge as limbs develop. Expression in the limb bud mesoderm is not maintained in the limbless mutant, which does not form an apical ridge. The mutant can be rescued by grafting normal ectoderm to the limb field. This leads to expression of the two homeo box genes in the mesoderm under the induced ridge. Phenocopies of eudiplopodia, which form an ectopic ridge on the limb bud, express the two genes under both ridges. When a quail ridge is grafted over nonexpressing mesoderm, a new site of expression is induced. Therefore, Hox-7 and Hox-8 depend on a functional ridge for their continued expression in the limb bud and can be induced by it.