Effect of biotin and a similar compound on succinic acid fermentation by Actinobacillus succinogenes in a chemically defined medium
Effect of biotin and a similar compound on succinic acid fermentation by Actinobacillus succinogenes in a chemically defined medium
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生物素和类似化合物对化学成分确定的培养基中产琥珀酸放线杆菌发酵琥珀酸的影响
DOI：
10.1016/j.bej.2012.08.016
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发表时间：
2012-12-15
影响因子：
3.9
通讯作者：
Chen, Jie-yu
中科院分区：
文献类型：
--
作者：
Xi, Yong-lan;Chen, Ke-quan;Chen, Jie-yu
建立了一种最小化学定义培养基（CDM），研究了生物素对琥珀酸放线杆菌nj113代谢的影响。培养基中含有四种氨基酸和维生素作为关键的生长因子。为了深入了解生物素的作用，我们添加了不同浓度的生物素，并研究了葡萄糖利用、细胞生长和琥珀酸浓度。在优化后的条件下，在3 L发酵罐中进行厌氧分批发酵，琥珀酸浓度为45.2 g/L，产率为90.4%。通过对生物素代谢机制的进一步研究，提出了生物素的代谢途径。当用5-氨基乙酰丙酸（5-ALA）代替生物素时，得到了相同的结果。我们认为5-ALA是生物发酵制备琥珀酸的一个很好的替代品。(C) 2012 Elsevier B.V.版权所有
A minimal chemically defined medium (CDM) was created and used to study the effect of biotin on the metabolism of Actinobacillus succinogenes NJ 113. The medium contained four amino acids and vitamins as key growth factors. To gain insight into the effect of biotin, different concentrations of this compound were added, and glucose utilisation, cell growth, and the succinic acid concentration were investigated. Anaerobic batch fermentation was carried out at optimised conditions in a 3 L fermentor with a succinic acid concentration of 45.2 g/L and a yield of 90.4%. A metabolic pathway was proposed after further study of the mechanism underlying the metabolism of biotin. The same results were obtained when 5-aminolevulinate (5-ALA) was used instead of biotin. We suggest that 5-ALA is a great alternative to biotin for the preparation of succinic acid by biological fermentation. (C) 2012 Elsevier B.V. All rights reserved.