Insights into the phylogenetic and taxonomy of philasterid ciliates (Protozoa, Ciliophora, Scuticociliatia) based on analyses of multiple molecular markers
Insights into the phylogenetic and taxonomy of philasterid ciliates (Protozoa, Ciliophora, Scuticociliatia) based on analyses of multiple molecular markers
复制标题
DOI：
10.1016/j.ympev.2012.04.008
复制
发表时间：
2012-08-01
影响因子：
4.1
通讯作者：
Song, Weibo
中科院分区：
文献类型：
--
作者：
Gao, Feng;Katz, Laura A.;Song, Weibo
Scuticociliates是一个丰富的物种组合，大多数未解决的系统发育关系，特别是在菲律宾。在目前的工作中，48个新的序列的三个连锁基因的特点和系统发育树的构建，以评估间和属内的关系的philoproids。结果表明：（1）组合的三基因树在节点上提供了比SSU-rDNA拓扑结构更高的分辨率;（2）Orchitophryidae科是非单系的，因为它分为两个部分，Paranophrys magna，Metanophrys sp.和Metanophrys sinensis在科水平上被指定为incertae sedis;（3）Uronematidae是非单系的，Homalogastra setosa被指定为incertae sedis;（4）拟尾丝科成为尾丝科的次异名，含A. Haemophila、Miamiensis avidus和Glauconema trihymene可能代表一个新的科：（5）支持将P. longum从P. longum中删除，并将其作为Uronema的次异名：（6）Uronema不是单系的，分子生物学分析表明Uronema sp. QD与Uronemella的共同祖先比其他Uronema的共同祖先更近，（7）Metanophrys是多系的;（8）两个极具争议的物种的多个样本，即。Mesanophrys pugettensis和M. chesapeakensis与M.（9）M. carcini和M.贪婪(C)2012 Elsevier Inc. All rights reserved.
Scuticociliates are a rich assemblage of species with mostly unresolved phylogenetic relationships, especially in the order Philasterida. In the present work, 48 new sequences for three linked genes are characterized and phylogenetic trees are constructed to assess the inter- and intra-generic relationships of philasterids. Results reveal the following: (1) the combined three-gene tree provides more resolution in nodes than in the SSU-rDNA topologies; (2) the family Orchitophryidae is non-monophyletic as it is split into two parts and Paranophrys magna, Metanophrys sp. and Metanophrys sinensis are designated incertae sedis at the familial level; (3) Uronematidae is non-monophyletic and Homalogastra setosa is designated incertae sedis; (4) Parauronematidae becomes a junior synonym of Uronematidae and the clade containing A. haemophila, Miamiensis avidus, and Glauconema trihymene might stand for a new family; (5) Parauronema being a junior synonym of Uronema is supported and P. longum should be removed from the genus Parauronema; (6) Uronema is not monophyletic and molecular analyses reveal that Uronema sp. QD shares a more recent common ancestor with Uronemella species than with other Uronema species; (7) Metanophrys is polyphyletic; (8) multiple samples of two highly controversial species, viz.. Mesanophrys pugettensis and M. chesapeakensis have identical ITS1-5.8S-ITS2 region sequence and we propose they should be synonymous with M. carcini, and (9) there may be cryptic species in M. carcini and M. avidus. (C) 2012 Elsevier Inc. All rights reserved.