Factors affecting the sporulation capacity during long-term storage of the aphid-pathogenic fungus Pandora neoaphidis grown on broomcorn millet.
Factors affecting the sporulation capacity during long-term storage of the aphid-pathogenic fungus Pandora neoaphidis grown on broomcorn millet.
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DOI：
10.1016/j.femsle.2005.03.015
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发表时间：
2005-04
影响因子：
2.1
通讯作者：
M. Feng;L. Hua
中科院分区：
文献类型：
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作者：
M. Feng;L. Hua
在黍上生长的蚜虫病原真菌 Pandora neoaphidis 比蚜虫尸体具有更大的产孢能力 (Cs)。通过在 20°C 下培养 15 天制备出含水量 (Cw) 为 48.7% 的孢子最多的培养物（32.0 × 104 孢子小米−1 粒），并用于研究长期储存过程中温度和湿度对 Cs 的影响。培养物用纸密封以保持环境湿度，用石蜡膜密封以保持饱和湿度，或保存在 85% 和 98% RH 的室中。在 5–20 °C 的 200 天储存期间对 Cwan 和 C 进行了监测。 5°C 下的纸密封培养物（Cw 为 21-25%）保存得最好，其 120 天的 Cs 与新鲜尸体相似。尽管活力较长，但 5 °C 下持续或可变的高 RH 会导致显着较高的 Cwan 和较低的 Cs。在 10°C 的条件下将培养物保存了 40 天。对于所有温度 (T) 状态，观测结果非常适合逻辑模型 Cs= 35.28/{1 + exp[−2.36 + (−0.003Cw+ 0.001CwT)t]} (r2= 0.95) 或 Cs= 35.55/[1 + exp(−2.33 + 0.001Cwt)] (r2= 0.93) at 5仅°C。 -0.003Cw+ 0.001CwTor 0.001Cw 在天数 (t) 内 Cs 的下降率凸显了 Cw 的主要效应。保存最佳的培养物和在 5 °C 下保存的风干尸体获得的每日 Cs 下降率惊人地相同。研究结果提出了一种可能的廉价方法来制备和储存大量磷。新甲虫。
Aphid-pathogenic fungus,Pandora neoaphidis, grown on broomcorn millet possesses greater sporulation capacity (Cs) than aphid cadavers. The most sporulating cultures (32.0 × 104spores millet−1grain) with water content (Cw) of 48.7% were prepared by incubation at 20 °C for 15 days and used to study the effect of temperature and humidity onCsduring long-term storage. Cultures were sealed with paper to retain ambient humidity, with parafilm for saturated humidity, or kept in 85% and 98% RH chambers. TheCwandCswere monitored during 200-day storage at 5–20 °C. The paper-sealed cultures at 5 °C, associated with 21–25% ofCw, were best preserved and their 120-dayCswas similar to that of the fresh cadavers. Consistently or variably high RH at 5 °C resulted in significantly higherCwand lowerCsdespite longer viability. The regimes at ?10 °C preserved the cultures for ?40 days. The observations fit well to the logistic modelCs= 35.28/{1 + exp[−2.36 + (−0.003Cw+ 0.001CwT)t]} (r2= 0.95) for all regimes of temperature (T) orCs= 35.55/[1 + exp(−2.33 + 0.001Cwt)] (r2= 0.93) at 5 °C only. The rate of decline ofCsof −0.003Cw+ 0.001CwTor 0.001Cwover days (t) highlights the primary effect ofCw. The dailyCs-decline rates obtained for the best-stored cultures and air-dried cadavers stored at 5 °C were surprisingly identical. The results suggest a possible cheap method for preparing and storing large quantities ofP. neoaphiodisinocula.