Quick separation and enzymatic performance improvement of lipase by ionic liquid-modified Fe3O4 carrier immobilization
Quick separation and enzymatic performance improvement of lipase by ionic liquid-modified Fe3O4 carrier immobilization
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DOI：
10.1007/s00449-018-1907-2
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发表时间：
2018-02
影响因子：
3.8
通讯作者：
Xia Jiaojiao;Zou Bin;Zhu Gangbin;W. Ping;Liu Zhenjiang
中科院分区：
文献类型：
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作者：
Xia Jiaojiao;Zou Bin;Zhu Gangbin;W. Ping;Liu Zhenjiang
为提高固定化猪胰脂肪酶的活性和稳定性，采用新型载体与特殊固定化方法相结合的方法。离子液体改性磁性Fe 3 O 4通过静电吸附法固定化酶后，酶的活性得到了提高。固定化酶（PPL-IM/BF 4-Fe3O4@CA）的酶活达到596 U/g PPL，通过静电吸附和包埋相结合的固定化方法，提高了载体与酶的结合力，进一步提高了固定化酶的效率和稳定性。PPL-IM/BF 4-Fe 3 O4@CA在重复第三次使用后的活性为78%。在室温下储存5天后，剩余活性为89%。研究了固定化酶的酶学性质和催化动力学，揭示了离子液体改性Fe 3 O 4对PPL的作用机理。通过XRD、FT-IR、SEM、TG等表征手段研究了离子液体对载体结构的影响。分析了静电吸附法和包埋法固定化PPL的机理和酶学性质。开发了一种新型高效的固定化PPL。
To promote the activity and stability of immobilized porcine pancreatic lipase (PPL), novel carrier was combined with special immobilization method. Enzymatic activity was enhanced after immobilized onto ionic liquid modified magnetic Fe3O4by electrostatic adsorption. Activity of immobilized enzyme (PPL-IM/BF4-Fe3O4@CA) reached 596 U/g PPL. Through the combination of electrostatic adsorption and embedding immobilization methods, we improve binding force between the carrier and enzyme, and further enhance the efficiency and stability of immobilized enzyme. The activity of PPL-IM/BF4-Fe3O4@CA after repeated third use was 78%. After storage at room temperature for 5 days, the residual activity was 89%. Enzymatic properties and catalytic kinetics of immobilized enzymes were studied, and the effect mechanism of ionic liquid modified Fe3O4on PPL was revealed. The effect of ionic liquid on the carrier structure was investigated by characterization of XRD, FT-IR, SEM and TG. The mechanism and enzymatic properties of immobilized PPL via electrostatic adsorption and embedding were analyzed. A novel and efficient immobilized PPL was developed.