A SIMPLIFIED PLAQUE-ASSAY FOR INFLUENZA-VIRUSES IN MADIN-DARBY KIDNEY (MDCK) CELLS
A SIMPLIFIED PLAQUE-ASSAY FOR INFLUENZA-VIRUSES IN MADIN-DARBY KIDNEY (MDCK) CELLS
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DOI：
10.1016/0166-0934(80)90020-8
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发表时间：
1980-01-01
影响因子：
3.1
通讯作者：
RABINOWITZ, S
中科院分区：
文献类型：
--
作者：
HUPRIKAR, J;RABINOWITZ, S
在胰酶中的MDCK[Madin-Darby犬肾]细胞中，只有在病毒吸附到单层时才加入各种甲型和乙型流感病毒的空斑。因此，无需添加蛋白水解酶即可采用传统的软琼脂覆盖层。提取效率与用胚胎卵作感染性判定相当。该方法简单、经济。
A variety of influenza A and B viruses plaque in MDCK [Madin-Darby canine kidney] cell in trypsin is added only at the time of viral adsorption to the monolayer. Therefore, a conventional soft-agar overlay can be employed without addition of proteolytic enzymes. Plaquing efficiency was comparable to that when embryonated eggs were used to determine infectivity. The method is simple and economical.