Ameliorative effect of naringenin on hyperglycemia-mediated inflammation in hepatic and pancreatic tissues of Wistar rats with streptozotocin-nicotinamide-induced experimental diabetes mellitus
Ameliorative effect of naringenin on hyperglycemia-mediated inflammation in hepatic and pancreatic tissues of Wistar rats with streptozotocin-nicotinamide-induced experimental diabetes mellitus
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DOI：
10.3109/10715762.2013.823643
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发表时间：
2013-10-01
影响因子：
3.3
通讯作者：
Geraldine, P.
中科院分区：
文献类型：
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作者：
Annadurai, T.;Thomas, P. A.;Geraldine, P.
在糖尿病（DM）中，持续的高血糖导致活性氧的产生，最终导致重要组织中氧化应激和炎症的增加。在本研究中，柚皮素对实验性链脲佐菌素（STZ）-烟酰胺诱导的DM中高血糖介导的炎症可能的改善作用进行了探索。在禁食过夜的Wistar大鼠（150-180 g）中通过腹腔注射STZ（50 mg/kg. B.w）和烟酰胺（110 mg/kg. B.w）;对照大鼠（n = 6）仅接受媒介物（0.5 ml的0.1 M冷柠檬酸盐缓冲液; pH 4.5）。一组糖尿病大鼠（n = 6）不处理，而另一组糖尿病大鼠（n = 6）接受柚皮素（50 mg/kg b.w./口服21天。此时，测量血液学指标（红细胞沉降率[ESR]、总白色血细胞[WBC]计数、白细胞分类百分比和血小板计数）。通过分别测量mRNA水平和表达的蛋白质水平以及这些组织中的总一氧化氮水平来确定肝脏和胰腺组织中炎症的基因和蛋白质生物标志物表达的显著改变。糖尿病大鼠血沉、白细胞总数、白细胞分类百分率和血小板计数均显著高于对照组;类似地，C-反应蛋白、促炎细胞因子核因子-在糖尿病大鼠的肝和胰腺组织样品中，κ B和诱导型一氧化氮合酶基因以及相应蛋白质的平均表达强度显著高于对照组。对照组大鼠。而经柚皮素治疗的糖尿病大鼠，其血液学指标、mRNA转录和蛋白质炎症指标均低于糖尿病大鼠。这些结果表明，柚皮素可能加剧高血糖介导的炎症实验STZ-烟酰胺诱导的糖尿病在Wistar大鼠。
In diabetes mellitus (DM), sustained hyperglycemia results in the generation of reactive oxygen species, ultimately leading to increased oxidative stress and inflammation in vital tissues. In the present study, possible ameliorative effects of naringenin on hyperglycemia-mediated inflammation in experimental streptozocin (STZ)-nicotinamide-induced DM were sought. DM was induced experimentally in overnight-fasted Wistar rats (150-180 g) by intra-peritoneal injection of STZ (50 mg/kg. b.w) and of nicotinamide (110 mg/kg. b.w); control rats (n = 6) received only vehicle (0.5 ml of 0.1 M of cold citrate buff er; pH 4.5). One group of diabetic rats (n = 6) was left untreated while another group of diabetic rats (n = 6) received naringenin (50 mg/kg b.w./day) orally for 21 days. At this time, hemotological indices (erythrocyte sedimentation rate [ESR], total white blood cell [WBC] count, differential WBC percentage, and platelet count) were measured. Significant alterations in expression of gene and protein biomarkers of inflammation in hepatic and pancreatic tissues were determined by measuring mRNA levels and the level of protein expressed, respectively, as was the total nitric oxide level in these tissues. Diabetic rats showed significantly higher mean ESR values, total WBC counts, differential WBC percentages, and platelet counts than those in control rats; similarly, mean mRNA levels of C-reactive protein, pro-inflammatory cytokine, nuclear factor-kappa B and inducible nitric oxide synthase genes and mean intensities of expression of the corresponding proteins in the hepatic and pancreatic tissue samples from diabetic rats significantly exceeded those in control rats. However, in diabetic rats treated with naringenin, the values of hematological, mRNA transcript and protein indices of inflammation were all lower than those in diabetic rats. These results suggest that naringenin possibly alleviates hyperglycemia-mediated inflammation in experimental STZ-nicotinamide-induced DM in Wistar rats.