Lipid composition and fluidity of plasma membranes isolated from corn (Zea mays L.) roots
Lipid composition and fluidity of plasma membranes isolated from corn (Zea mays L.) roots
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DOI：
10.1006/abbi.2000.2224
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发表时间：
2001-03-01
影响因子：
3.9
通讯作者：
Lüthje, S
中科院分区：
文献类型：
--
作者：
Bohn, M;Heinz, E;Lüthje, S
尽管几种植物的脂类分析结果已有多年，但对玉米根质膜的完整表征仍然缺乏。本研究详细分析了用相分离方法分离的玉米(Zea Mays L，Zea Mays)根质膜的各种脂类物质和膜流动性的特征。磷脂(43.9mol%)、甾醇(40.8mol%)和以葡萄糖脑苷形式存在的鞘磷脂(6.8mol%)是主要的脂类。豆甾醇(19.8摩尔%)、菜籽甾醇(13.0摩尔%)、磷脂酰胆碱(15.8摩尔%)和磷脂酰乙醇胺(14.2摩尔%)是最普遍存在的单项脂肪。葡萄糖脑苷中羟基脂肪酸占总脂肪酸的80.9%，超长链脂肪酸占总脂肪酸的78%。羟基花生四烯酸(20：0h)和羟基甘油酸(24：0h)含量最高，玉米根质膜中的葡萄糖脑苷几乎不含不饱和脂肪酸。在磷脂中，只有磷脂酰丝氨酸显示出很高比例的超长链脂肪酸(如山毛酸和甘油酸)。膜流动性是通过荧光各向异性来估计的，由于玉米根的质膜中含有较高的甾醇含量，因此质膜相对刚性，(C)2001学术出版社。
Although the results of lipid analyses from several plant species have been available for many years a complete characterization of the corn root plasma membrane is still lacking. The present study provides a detailed analysis of individual lipids and a characterization of the membrane fluidity of corn (Zea mays L,) root plasma membranes isolated by phase-partitioning. Phospholipids (43.9 mol%), sterols (40.8 mol%), and sphingolipids in the form of glucocerebroside (6.8 mol%) constitute the major lipid classes. Stigmasterol (19.8 mol%), campesterol (13.0 mol%), phosphatidylcholine 15.8 mol%), and phosphatidylethanolamine (14.2 mol%) represent the most ubiquitous individual lipids. Hydroxy fatty acids make up 80.9 mol% and very long chain fatty acids are almost 78% of fatty acids in glucocerebroside. Hydroxy arachidic acid (20:0 h) and hydroxy lignoceric acid (24:0 h) are most prominent and glucocerebroside from corn root plasma membranes contains virtually no unsaturated fatty acids. Among the phospholipids only phosphatidylserine displayed a high proportion of very long chain fatty acids (e.g., behenic and lignoceric acid). Membrane fluidity was estimated by fluorescence anisotropy, Due to the high sterol content the plasma membrane of corn roots is relatively rigid, (C) 2001 Academic Press.