A bovine acellular scaffold for vocal fold reconstruction in a rat model.
A bovine acellular scaffold for vocal fold reconstruction in a rat model.
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用于大鼠模型声带重建的牛脱细胞支架。
DOI：
10.1002/jbm.a.32279
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发表时间：
2010
期刊：
影响因子：
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通讯作者：
Pawlowski,KarenS
中科院分区：
文献类型：
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作者：
Xu,ChetC;Chan,RogerW;Weinberger,DebraG;Efune,Guy;Pawlowski,KarenS
本研究通过建立大鼠声带损伤模型，研究了牛声带固有层脱细胞异种支架的体内宿主反应，以及该支架对组织重建的潜力。在20只大鼠的双侧声带后部造成伤口，单侧植入脱细胞牛软骨支架，对侧声带作为对照。分别于术后3天、7天、1个月、3个月人道处死大鼠，取喉冠状切片进行组织学检查。通过数字图像分析定量关键基质蛋白的表达，包括I型胶原、III型胶原、弹性蛋白、纤连蛋白、透明质酸和糖胺聚糖（GAGs）。在伤口修复的急性期（术后3天和7天）观察到支架植入物中宿主炎性细胞和宿主成纤维细胞的显著浸润。植入声带的I型胶原、III型胶原和GAG的平均相对密度在3天后显著高于对照组，随后在3个月内逐渐降低。组织学结果显示，支架在3个月内明显降解，无纤维化组织形成或钙化。这些初步研究结果表明，牛脱细胞支架可能是一个潜在的异种移植声带再生。© 2009 Wiley Periodicals，Inc.生物医学材料研究杂志，2010年
With a rat model of vocal fold injury, this study examined thein vivohost response to an acellular xenogeneic scaffold derived from the bovine vocal fold lamina propria, and the potential of the scaffold for constructive tissue remodeling. Bilateral wounds were created in the posterior vocal folds of 20 rats, and bovine acellular scaffolds were implanted into the wounds unilaterally, with the contralateral vocal folds as control. The rats were humanely sacrificed after 3 days, 7 days, 1 month, and 3 months, and the coronal sections of their larynges were examined histologically. Expressions of key matrix proteins including collagen I, collagen III, elastin, fibronectin, hyaluronic acid, and glycosaminoglycans (GAGs) were quantified with digital image analysis. Significant infiltration of host inflammatory cells and host fibroblasts in the scaffold implant was observed in the acute stage of wound repair (3 days and 7 days postsurgery). The mean relative densities of collagen I, collagen III, and GAGs in the implanted vocal folds were significantly higher than those in the control after 3 days, followed by gradual decreases over 3 months. Histological results showed that the scaffolds were apparently degraded by 3 months, with no fibrotic tissue formation or calcification. These preliminary findings suggested that the bovine acellular scaffold could be a potential xenograft for vocal fold regeneration. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010