A Listeria monocytogenes pentapeptide is presented to cytolytic T lymphocytes by the H2-M3 MHC class Ib molecule
A Listeria monocytogenes pentapeptide is presented to cytolytic T lymphocytes by the H2-M3 MHC class Ib molecule
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DOI：
10.1016/s1074-7613(00)80311-8
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发表时间：
1996-07-01
期刊：
影响因子：
32.4
通讯作者：
Pamer, EG
中科院分区：
文献类型：
--
作者：
Gulden, PH;Fischer, P;Pamer, EG
MHC Ia类分子的多态性严重限制了针对细胞内病原体的疫苗开发。通过MHC Ib类分子的抗原呈递，通常在不同个体之间是保守的，可以绕过这个障碍。在此，我们使用串联质谱法来鉴定由H2-M3 MHC Ib类分子呈递给T淋巴细胞的单核细胞增生李斯特菌五肽抗原。该肽在N末端含有N-甲酰基甲硫氨酸，并且仅含有疏水性氨基酸。H-2（d）、H-2（B）和H-2（k）单倍型的小鼠在感染单核细胞增生李斯特菌后对该肽产生应答。鉴定由MHC Ib类分子呈递的抗原是可行的，并且可以为相对不受限制的疫苗开发提供机会。
Polymorphism of MHC class Ia molecules severely constrains vaccine development against intracellular pathogens. Antigen presentation by MHC class Ib molecules, which are generally conserved between different individuals, may circumvent this obstacle. Herein, we use tandem mass spectrometry to identify a Listeria monocytogenes pentapeptide antigen that is presented to T lymphocytes by the H2-M3 MHC class Ib molecule. The peptide contains N-formyl methionine at the N terminus and exclusively hydrophobic amino acids. Mice of the H-2(d), H-2(b), and H-2(k) haplotypes respond to this peptide upon infection with Listeria monocytogenes. Identification of antigens presented by MHC class Ib molecules is feasible and may provide opportunities for relatively unrestricted vaccine development.