A DNA rotary nanodevice operated by enzyme-initiated strand resetting.
A DNA rotary nanodevice operated by enzyme-initiated strand resetting.
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DOI：
10.1039/d3cc05487j
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发表时间：
2023-12
影响因子：
4.9
通讯作者：
A. Chandrasekaran
中科院分区：
文献类型：
--
作者：
A. Chandrasekaran
对外部刺激有响应的DNA纳米结构已在生物传感、药物递送和分子计算等多个领域得到应用。在单次操作中使用不同类型的刺激为核酸纳米结构的重新配置提供了另一层控制。这项工作展示了利用一种核糖核酸酶使基于平行错排交叉（PX）DNA的核酸纳米装置“复位”，以及利用特定的DNA输入将结构“重置”为并列DNA（JX2）构型，从而导致螺旋结构域旋转180°。这种操作在转化应用中是有用的，在这些应用中，可以设计DNA纳米结构使其根据不止一种刺激进行重新配置。
DNA nanostructures that respond to external stimuli have found applications in several areas such as biosensing, drug delivery and molecular computation. The use of different types of stimuli in a single operation provides another layer of control for the reconfiguration of nucleic acid nanostructures. This work demonstrates the use of a ribonuclease to "unset" a nucleic acid nanodevice based on the paranemic crossover (PX) DNA and specific DNA inputs to "reset" the structure into a juxtaposed DNA (JX2) configuration, resulting in a 180° rotation of the helical domains. Such operations would be useful in translational applications where DNA nanostructures can be designed to reconfigure on the basis of more than one stimulus.