Phagocytosing neutrophils produce and release high amounts of the neutrophil-activating peptide 1/interleukin 8.
Phagocytosing neutrophils produce and release high amounts of the neutrophil-activating peptide 1/interleukin 8.
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DOI：
10.1084/jem.173.3.771
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发表时间：
1991-03-01
影响因子：
15.3
通讯作者：
Baggiolini, M
中科院分区：
文献类型：
--
作者：
Bazzoni, F;Cassatella, M A;Rossi, F;Ceska, M;Dewald, B;Baggiolini, M
经IgG活化的酵母吞噬后，中性粒细胞白细胞（多形核白细胞[PMN]）表达高水平的中性粒细胞活化肽1/白细胞介素8 (NAP-1/IL-8) mRNA，在3-5 h后达到峰值，在18 h后仍然升高。脂多糖（LPS）也有类似的效果，但在数量上不那么显著。在吞噬后，而不是暴露于LPS后，PMN逐渐向培养基中释放大量的NAP-1/IL-8 （18 h内18.6-50 ng/ml）。释放的肽具有生物活性，表明暴露于等量培养上清的PMN中胞质游离钙的短暂升高，以及重组NAP-1/IL-8对细胞的预刺激脱敏。通过在吞噬入侵微生物的位点产生NAP-1/IL-8， PMN可以促进新的防御细胞的招募。
After phagocytosis of yeast opsonized with IgG, neutrophil leukocytes (polymorphonuclear leukocytes [PMN]) expressed high levels of neutrophil-activating peptide 1/interleukin 8 (NAP-1/IL-8) mRNA, which peaked after 3-5 h and were still elevated after 18 h. A similar but quantitatively less prominent effect was obtained with lipopolysaccharide (LPS). After phagocytosis, but not after exposure to LPS, the PMN progressively released considerable amounts of NAP-1/IL-8 into the culture medium (18.6-50 ng/ml in 18 h). The peptide released was biologically active, as indicated by the transient elevation of cytosolic-free calcium in PMN exposed to aliquots of the culture supernatants, and desensitization by prestimulation of the cells with recombinant NAP-1/IL-8. By producing NAP-1/IL-8 at sites where they phagocytose invading microorganisms, PMN could enhance the recruitment of new defense cells.