Photoperiod regulates the differential expression of KiSS-1 and GPR54 in various tissues and sexes of striped hamster
Photoperiod regulates the differential expression of KiSS-1 and GPR54 in various tissues and sexes of striped hamster
复制标题
光周期调节条纹仓鼠不同组织和性别中KiSS-1和GPR54的差异表达。
DOI：
10.4238/2015.october.29.10
复制
发表时间：
2015-01-01
影响因子：
0.4
通讯作者：
Xu, L. X.
中科院分区：
文献类型：
--
作者：
Li, S. N.;Xue, H. L.;Xu, L. X.
为了研究光周期如何影响 KiSS-1/G 蛋白偶联受体 54 (GPR54) 的表达，在光周期处理和褪黑激素注射后，对条纹仓鼠的下丘脑、卵巢和睾丸进行了研究。我们发现条纹仓鼠血液中褪黑激素的浓度与处理的光周期一致，并且光周期对KiSS-1表达有显着影响，但光周期对GPR54表达的影响在不同组织和性别之间存在差异。雄性和雌性条纹仓鼠性腺中KiSS-1的相对表达量显着高于下丘脑，而雄性和雌性条纹仓鼠性腺和下丘脑中GPR54的差异不一致。注射褪黑激素的条纹仓鼠中，雄性和雌性条纹仓鼠性腺和下丘脑中KiSS-1的表达水平显着降低；性腺中GnRH的表达水平也显着降低；下丘脑中GPR54的表达增加。在下丘脑中,KiSS-1与GnRH表达呈显着正相关,而GPR54与GnRH表达呈负相关。这些结果表明光周期-褪黑激素-KiSS-1-GnRH通路在条纹仓鼠的季节性繁殖机制中发挥着重要作用。此外，光周期或褪黑激素可能不是 GPR54 表达的直接调节因素。
In order to investigate how photoperiod affects the expression of KiSS-1/G-protein-coupled receptor 54 (GPR54), the hypothalamus, the ovaries and testicles of the striped hamster were studied following photoperiod treatment and melatonin injection. We found that the melatonin concentration in the blood of the striped hamster was consistent with the treated photoperiods and that the photoperiod had significant effects on KiSS-1 expression, but the effect of photoperiod on GPR54 expression differed among tissues and sexes. The relative expression of KiSS-1 in the gonads of the male and female striped hamster was significantly higher than that in the hypothalamus, while the difference in GPR54 between the gonads and hypothalamus was inconsistent between the male and female striped hamster. In the striped hamster injected with melatonin, the expression level of KiSS-1 in the gonads and hypothalamus of the male and female striped hamster was significantly decreased; the expression level of GnRH in the gonads was also significantly decreased; the expression of GPR54 in the hypothalamus was increased. In the hypothalamus, a significant positive correlation existed between KiSS-1 and GnRH expression, while a negative correlation was observed between GPR54 and GnRH expression. These results suggest that the photoperiod-melatonin-KiSS-1-GnRH pathway plays an important role in the seasonal reproduction mechanism of the striped hamster. Additionally, photoperiod or melatonin may not be a direct regulatory factor of GPR54 expression.