Average Shape Standard Atlas for the Adult Drosophila Ventral Nerve Cord
Average Shape Standard Atlas for the Adult Drosophila Ventral Nerve Cord
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DOI：
10.1002/cne.22346
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发表时间：
2010-07-01
影响因子：
2.5
通讯作者：
Duch, Carsten
中科院分区：
文献类型：
--
作者：
Boerner, Jana;Duch, Carsten
神经解剖学受益于神经结构的量化，即，神经元，电路和大脑部分，在一个共同的参考系统。最近成像技术的改进和计算能力的提高使得创建基于Web的数据库成为可能，这些数据库可以作为整合解剖数据的通用平台。本研究建立了果蝇腹神经索（VNC）的标准平均形状图谱。该图谱允许将形态、发育和遗传数据注册到一个定量3D参考系统中。该标准品是基于24个整体封片制备物中标记的平均成年果蝇VNC神经元，该制备物具有识别果蝇Bruchpilot蛋白（Brp）的市售抗体（nc 82）。对于标准化过程，不需要大脑解剖学的专业知识，并且全局阈值以及简单的仿射和弹性配准过程最小化了用户交互。成功的注册证明为束和连合，基因表达模式，和单个神经元的几何重建。任何用抗Brp复染的结构都可以注册到标准品中，从而可以快速比较来自不同实验室的数据。此外，标准变换可以应用于灰度图像数据，使得可以在标准化的3D参考坐标内分析与anti-Brp共标记的任何共焦图像堆栈。这允许创建推定的神经连接图和比较来自不同制剂的表达模式。组织学方法、分割和配准程序的标准和协议将在网上公布。神经学比较杂志518：2437-2455，2010. (C)2010 Wiley-Liss，Inc.
Neuroanatomy benefits from quantification of neural structures, i.e., neurons, circuits, and brain parts, within a common reference system. Recent improvements in imaging techniques and increased computational power have made the creation of Web-based databases possible, which serve as common platforms for incorporating anatomical data. This study establishes a standard average shape atlas for the ventral nerve cord (VNC) of Drosophila melanogaster. This atlas allows for the registration of morphological, developmental, and genetic data into one quantitative 3D reference system. The standard is based on an average adult Drosophila VNC neuropil as labeled in 24 whole-mount preparations with the commercially available antibody (nc82) recognizing the Drosophila Bruchpilot protein (Brp). For the standardization procedure no expert knowledge of brain anatomy is required and global thresholding as well as straightforward affine and elastic registration procedures minimize user interactions. Successful registration is demonstrated for tracts and commissures, gene expression patterns, and geometric reconstructions of individual neurons. Any structure that is counterstained with anti-Brp can be registered into the standard, allowing for fast comparison of data from different experiments and different laboratories. In addition, standard transformations can be applied to gray scale image data, so that any confocal image stack that is colabeled with anti-Brp can be analyzed within standardized 3D reference coordinates. This allows for the creation of putative neural connectivity maps and the comparison of expression patterns derived from different preparations. The standard and protocols for histological methods, segmentation, and registration procedures will be made available on the Web. J. Comp. Neurol. 518:2437-2455, 2010. (C) 2010 Wiley-Liss, Inc.