Expression and localization of mesothelin in developing rat pancreas
Expression and localization of mesothelin in developing rat pancreas
复制标题
间皮素在发育中的大鼠胰腺中的表达和定位
DOI：
10.1111/j.1440-169x.2008.01047.x
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发表时间：
2008-09-01
影响因子：
2.5
通讯作者：
De, Wei
中科院分区：
文献类型：
--
作者：
Hou, Liang-Qin;Wang, Yin-He;De, Wei
为了确定调节胰腺发育的遗传网络，我们使用高密度微阵列（Affymetrix）来生成5个生物学重要发育阶段的大鼠胰腺转录谱：胚胎12.5天（E12.5）， E15.5, E18.5，出生后第0天（P0）和成年。在妊娠后期，当胰岛结构和功能逐渐形成时，许多基因显著高表达。我们进一步研究了其中一个基因间皮素的表达和定位。逆转录聚合酶链反应和Western blot分析显示，在妊娠后期至出生后2-3周间皮素mRNA和蛋白水平较高，在成年大鼠胰腺中表达水平相对较低，但可检测到。免疫定位表明，间皮素不仅存在于胰岛细胞中，也存在于发育中的大鼠胰腺间质中。瞬时间皮素的表达伴随着胰岛结构的形成、重塑和成熟。这些结果表明，间皮素在发育中的大鼠胰腺中是动态表达的，并且可能参与了大鼠胰腺发育过程中的一些发育事件。
To define a genetic network that regulates development of the pancreas, we used high-density microarray (Affymetrix) to generate transcriptional profiles of rat pancreas from five biologically significant stages of development: embryonic day 12.5 (E12.5), E15.5, E18.5, postnatal day 0 (P0) and adult. Many genes were notably highly expressed in the later gestation when islet architecture and function are gradually forming. The expression and localization of mesothelin, one of these genes, was further examined. Reverse transcription-polymerase chain reaction and Western blot analysis revealed that mRNA and protein levels of mesothelin were high from later gestation to 2-3 weeks after birth, and with relatively low but detectable expression levels in adult rat pancreas. Immunolocalization indicated that mesothelin localized not only in islet beta-cells but also in the mesenchyme of developing rat pancreas. Transient mesothelin expression was concomitant with the development of islets architecture formation, remodeling and maturation. These findings indicate that mesothelin is dynamically expressed in the developing rat pancreas and that mesothelin might be involved in some developmental events during development of rat pancreas.