Immunofluorescent identification of endogenous neurotransmitter content in Golgi-impregnated neurons.
Immunofluorescent identification of endogenous neurotransmitter content in Golgi-impregnated neurons.
复制标题
高尔基体浸渍神经元中内源性神经递质含量的免疫荧光鉴定。
DOI：
10.1016/0165-0270(93)90139-i
复制
发表时间：
1993
影响因子：
3
通讯作者：
Yazulla,S
中科院分区：
文献类型：
--
作者：
Sherry,DM;Yazulla,S
采用高尔基浸渍/免疫细胞化学相结合的方法来鉴定形态学特征神经元的内源性神经递质含量。用厚树脂切片对高尔基浸渍蜥蜴的视网膜无突细胞进行了形态学表征。重新安装感兴趣的细胞，在1 μm厚度处切除，并进行包埋后免疫荧光检查，以观察氨基酸神经递质γ -氨基丁酸（GABA）或甘氨酸。双标记细胞在明亮视场照射下通过细胞质中不透明的高尔基体沉积进行鉴定，在紫外线照射下通过细胞核免疫荧光进行鉴定。27个高尔基浸渍的无毛细胞，表现出gaba免疫反应（GABA-IR）细胞的形态特征，进行GABA-IR检测；21个显示双贴标。使用高尔基/免疫细胞化学方法也鉴定了甘氨酸- ir无分泌细胞。这种双重标记程序允许快速评估内源性神经递质含量在大样本的形态学特征的神经元。
A combined Golgi-impregnation/immunocytochemistry procedure was developed to identify the endogenous neurotransmitter content of morphologically characterized neurons. Golgi-impregnated retinal amacrine cells in the lizardAnolis carolinensiswere characterized morphologically in thick resin sections. Cells of interest were remounted, resectioned at 1 μm thickness and subjected to a postembedding immunofluorescene procedure to visualize the amino acid neurotransmitters gamma-aminobutyric acid (GABA) or glycine. Double-labeled cells were identified by opaque Golgi deposits in the cytoplasm under bright-field illumination and nuclear immunofluorescence under ultraviolet illumination. Twenty-seven Golgi-impregnated amacrine cells, exhibiting morphological features of GABA-immunoreactive (GABA-IR) cells, were tested for GABA-IR; 21 showed double labeling. Glycine-IR amacrine cells also were identified using the Golgi/immunocytochemistry procedure. This double-labeling procedure allows rapid assessment of endogenous neurotransmitter content in large samples of morphologically characterized neurons.