Acute exercise activates local bioactive androgen metabolism in skeletal muscle
Acute exercise activates local bioactive androgen metabolism in skeletal muscle
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DOI：
10.1016/j.steroids.2009.12.002
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发表时间：
2010-03-01
期刊：
影响因子：
2.7
通讯作者：
Akimoto, Takayuki
中科院分区：
文献类型：
--
作者：
Aizawa, Katsuji;Iemitsu, Motoyuki;Akimoto, Takayuki
雄激素，如睾酮，在靶组织的各种生理过程中起着重要的调节作用。最近，我们报道了骨骼肌中存在类固醇生成酶，并调节运动后局部睾酮的产生。睾酮通过5α-还原酶转化为具有生物活性的雄激素代谢产物二氢睾酮(DHT)。然而，目前还不清楚运动是否会刺激男女骨骼肌局部的生物活性雄激素代谢。在本研究中，我们研究了单次运动前后大鼠骨骼肌中脱氢表雄酮(DHEA)、游离睾酮、脱氢表雄酮、类固醇生成相关酶5α-还原酶和雄激素受体(AR)水平的性别差异。基础肌肉游离睾酮和DHT水平男性高于女性，而DHEA水平在性别之间没有差异。运动后，两种性别的肌肉DHEA、游离睾酮和DHT水平都有所增加。5-α-还原酶和AR转录本及蛋白的性别差异无统计学意义，运动后5-α-还原酶在两性间的表达均显著增加。最后，运动后AR在雌性大鼠中的表达显著高于雄性大鼠，而在雄性大鼠中则没有。这些数据表明，急性运动增强了男女骨骼肌局部的生物活性雄激素代谢。(C)2009 Elsevier Inc.保留所有权利。
Androgens, Such as testosterone, play important roles in regulation of diverse physiological process of target tissues. Recently, we reported that steroidogenic enzymes exist in skeletal Muscle and regulate local production of testosterone in response to exercise. Testosterone is transformed into a bioactive androgen metabolite, dihydrotestosterone (DHT) by 5 alpha-reductase. However, it is unclear whether exercise stimulates local bioactive androgen metabolism in the skeletal muscle in both sexes. In the present study, we examined sex differences in the levels of dehydroepiandrosterone (DHEA), free testosterone, DHT, and steroidogenesis-related enzymes 5 alpha-reductase and androgen receptor (AR) in rat's skeletal muscle before and after a single bout of exercise. Basal muscular free testosterone and DHT levels were higher in males than females, whereas the levels of DHEA did not differ between the sexes. Muscular DHEA, free testosterone, and DHT levels were increased in both sexes after the exercise. There were no differences of 5 alpha-reductase and AR transcripts and proteins between the sexes, and the expression of 5 alpha-reductase was significantly increased in both sexes after the exercise. Finally, the expression of AR was significantly higher in female rats, but not in males after the exercise. These data Suggest that acute exercise enhances the local bioactive androgen metabolism in the skeletal Muscle of both sexes. (C) 2009 Elsevier Inc. All rights reserved.