Sexing of carcass remains of the Sika deer (Cervus nippon) using PCR amplification of the Sry gene
Sexing of carcass remains of the Sika deer (Cervus nippon) using PCR amplification of the Sry gene
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DOI：
10.1292/jvms.60.713
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发表时间：
1998-06-01
影响因子：
1.2
通讯作者：
Ohtaishi, N
中科院分区：
文献类型：
--
作者：
Takahashi, M;Masuda, R;Ohtaishi, N
为了鉴定西卡鹿（Cervus nippon）尸体遗骸的性别，我们改进了聚合酶链反应（PCR）技术，扩增了哺乳动物Y染色体上雄性特异DNA区域西卡鹿Sry。从这里获得的Sry区域的核苷酸序列，新设计了能够扩增较短Sry区域的PCR引物MT 1和MT 2，并使用微卫星基因座作为阳性对照。使用这些引物，96 109性别未知的小鹿（88%，96/109）成功地进行了性别鉴定（46名男性和50名女性），无论尸体在现场发现的条件。本研究结果和方法对西卡种群死亡规律的研究具有重要意义。
In Order to determine the sex of carcass remains of the Sika deer (Cervus nippon), we improved a polymerase chain reaction (PCR) technique for amplification of the Sika deer Sry, a male-specific DNA region on the mammalian Y chromosome. From the nucleotide sequence of the Sry region obtained here, PCR primers, MT1 and MT2, capable of amplifying a shorter Sry region were newly designed, and a microsatellite locus was used as a positive control. Using these primers, 96 of 109 sex-unknown fawns (88%, 96/109) were successfully sexed (46 males and 50 females) regardless of the conditions of carcasses found in the field. The results and the methodology could greatly contribute to the study of the mortality pattern of the Sika deer population.