Morroniside on anti-inflammation activities in rats following acute myocardial infarction
Morroniside on anti-inflammation activities in rats following acute myocardial infarction
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莫诺苷对急性心肌梗死大鼠的抗炎作用
DOI：
10.4196/kjpp.2018.22.1.17
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发表时间：
2017-12
影响因子：
2
通讯作者：
Wang Wen
中科院分区：
文献类型：
--
作者：
Yu Bangxing;Zhang Guoxing;An Yi;Wang Wen
我们之前的研究已经证实了莫罗尼苷具有神经保护作用。然而，Morroniside对心肌的作用尚不清楚。大鼠以10%水合氯醛(0.35~0.4mL/kg)麻醉，结扎冠状动脉前降支(LAD)复制急性心肌梗死(AMI)模型。急性心肌梗死后连续3d灌胃给药，剂量分别为45、90、180 mg/kg。用商品化试剂盒检测血清乳酸脱氢酶(LDH)和心肌肌钙蛋白T(CTnT)活性。用免疫印迹法检测心肌组织中IL-6、IL-1β和肿瘤坏死因子-α的表达。观察到72小时后，莫罗尼苷治疗组大鼠Q(Q)波幅显著下降，并能降低急性心肌梗死大鼠血清乳酸脱氢酶和肌钙蛋白T水平。此外，我们还观察到莫诺苷降低了心肌中IL-6、IL-1β和肿瘤坏死因子-α的表达。综上所述，我们的研究结果表明，莫诺苷对急性心肌梗死大鼠具有有效的抗炎作用。
Our previous studies have confirmed that morroniside has neuroprotective effects. However, the effects of morroniside on cardiac myocardium remain unknown. Rats were anaesthetized with 10% chloral hydrate (0.35~0.4 mL/kg) and an acute myocardial infarction (AMI) was induced by ligating the anterior descending coronary artery (LAD). Following AMI, morroniside was administered intragastrically for 3 consecutive days at doses of 45, 90 and 180 mg/kg, respectively. Lactate dehydrogenase (LDH) and cardiac troponin T (cTnT) activities in AMI rats in the serum were detected with commercial kits. The expression of IL-6, IL-1β and TNF-α in myocardium was detected by Western blotting analysis. We observed a significant decline in the Q(q) wave amplitude in morroniside-treated rats after 72 h. Additionally, treatment of morroniside decreased the levels of LDH and cTnT in AMI rats. We also observed that morroniside reduced the expression of IL-6, IL-1β and TNF-α in myocardium. Taken together, our findings demonstrate that morroniside had effective anti-inflammatory properties in AMI rats.
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DOI：
10.1136/heartjnl-2013-303648
发表时间：
2013-09
期刊：
Heart (British Cardiac Society)
影响因子：
--
作者：
Padfield GJ;Din JN;Koushiappi E;Mills NL;Robinson SD;Cruden Nle M;Lucking AJ;Chia S;Harding SA;Newby DE
通讯作者：
Newby DE
DOI：
10.1038/nrm3619
发表时间：
2013-08
期刊：
Nature reviews. Molecular cell biology
影响因子：
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作者：
通讯作者：
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DOI：
10.1007/s11596-012-1033-3
发表时间：
2012-10
期刊：
Journal of Huazhong University of Science and Technology [Medical Sciences]
影响因子：
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作者：
Wen Wu;Linxiao Huang;Jiang-xia Zhang;Yu Gao;Yali Yang
通讯作者：
Wen Wu;Linxiao Huang;Jiang-xia Zhang;Yu Gao;Yali Yang
影响因子：
21.3
作者：
D'Uva, Gabriele;Aharonov, Alla;Tzahor, Eldad
通讯作者：
Tzahor, Eldad
DOI：
10.36503/chcmj1(1)-03
发表时间：
2004
期刊：
Procedia Engineering
影响因子：
--
作者：
A. Maseri;D. Cianflone
通讯作者：
A. Maseri;D. Cianflone