PRESENCE OF HIGHLY REPETITIVE DNA-SEQUENCES IN TRIBOLIUM FLOUR-BEETLES
PRESENCE OF HIGHLY REPETITIVE DNA-SEQUENCES IN TRIBOLIUM FLOUR-BEETLES
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DOI：
10.1038/hdy.1993.1
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发表时间：
1993-01-01
期刊：
影响因子：
3.8
通讯作者：
HEWITT, GM
中科院分区：
文献类型：
--
作者：
JUAN, C;VAZQUEZ, P;HEWITT, GM
用Sau3AI、Taqi和ClaI限制性内切酶对7种雷波氏菌(鞘翅目)的基因组DNA进行酶切，发现这些种中存在大量的高度重复的DNA序列。在弗里曼赤霉菌中，这些序列与166个碱基的富含A-T的卫星单体(70.5%)串联重复，占总基因组的31%，原位杂交证明它位于着丝粒染色体区域。由于频繁的反向重复序列的存在，该序列有可能形成二级结构，如茎或十字形。赤拟谷盗、Anaphe和T.madens与自由毛滴虫卫星DNA序列具有同源性，而Confusum、T.audax、T.brevicornis和黄粉虫、黄粉虫等黄粉虫则没有同源序列。根据斑点杂交法得到的高度重复序列的同源性和丰富性，结合化学分类学和核学特征，构建了7个研究种的系统发育树。
Digestion of genomic DNA from seven species of Tribolium (Coleoptera) with Sau3AI, TaqI and ClaI restriction enzymes shows the presence of remarkable amounts of highly repetitive DNA sequences in these species. In Tribolium freemani the sequences are tandemly repeated with a satellite monomer of 166 bp, A-T rich (70.5 per cent), representing 31 per cent of the total genome and located in centromeric chromosome areas as demonstrated by in situ hybridization. The sequence has the potential to form secondary structures such as stems or cruciforms due to the presence of frequent inverted repeats. Tribolium castaneum, T. anaphe and T. madens show homologous sequences to T. freemani satellite DNA but T. confusum, T. audax, T. brevicornis and other tenebrionid beetles, such as Tenebrio molitor and Misolampus goudoti, do not. A phylogenetic dendrogram, based on the homology and abundance of highly repetitive sequences deduced by dot-blot hybridization, chemotaxonomic and karyological characters, is proposed for the seven studied species of Tribolium.