An action spectrum for UV-induced attachment of V79 Chinese hamster cells to a substratum.
An action spectrum for UV-induced attachment of V79 Chinese hamster cells to a substratum.
复制标题
紫外线诱导 V79 中国仓鼠细胞附着于基质的作用谱。
DOI：
10.1080/09553009314551621
复制
发表时间：
1993
影响因子：
2.6
通讯作者：
J. Moan
中科院分区：
文献类型：
--
作者：
H. Bånrud;K. Berg;T. Platou;J. Moan
当单层细胞生长暴露于紫外线辐射（UV）时，它们与基质的结合强度增加。测定了这种UV诱导结合的作用光谱，使用胰蛋白酶-EDTA分离细胞所需的时间作为结合强度的量度。该作用谱与同样测定的细胞灭活作用谱显著不同。在最短波长（297/302、313 nm）下，需要致死注量才能诱导可测量的结合，而在最长波长（365、405 nm）下，完全非致死注量诱导强烈且持久的结合。因此，不同的发色团参与了这两个过程：虽然DNA可能是细胞失活的主要发色团，但其他未识别的发色团可能对诱导细胞与基质结合增加更重要。
When cells growing in monolayers are exposed to ultraviolet radiation (UV) their binding to the substratum is increased in strength. An action spectrum for such UV-induced binding was determined, using the time needed for trypsin-EDTA to detach the cells as a measure of the binding strength. This action spectrum was significantly different from the action spectrum for cell inactivation, which was also determined. At the shortest wavelengths (297/302, 313 nm) lethal fluences were needed to induce measurable binding while at the longest wavelengths (365, 405 nm) completely nonlethal fluences induced strong and persistent binding. Thus, different chromophores are involved in the two processes: while DNA may be the main chromophore for cell inactivation, other and unidentified chromophores may be more important for induction of increased cell binding to the substratum.
血清、胰蛋白酶和细胞形状影响中国仓鼠 V79 细胞单层和球体的 X 射线敏感性，但不影响细胞与细胞之间的接触。
影响因子：
3.4
作者：
Reddy,NM;Lange,CS
通讯作者：
Lange,CS