Regulation of Arabidopsis thaliana seed dormancy and germination by 12-oxo-phytodienoic acid.
Regulation of Arabidopsis thaliana seed dormancy and germination by 12-oxo-phytodienoic acid.
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DOI：
10.1093/jxb/erw028
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发表时间：
2016-04
影响因子：
6.9
通讯作者：
Graham IA
中科院分区：
文献类型：
--
作者：
Dave A;Vaistij FE;Gilday AD;Penfield SD;Graham IA
我们证明，12-氧代植物二烯酸的行为与阿坝在一个复杂的调控网络，以促进种子休眠和抑制萌发拟南芥。我们以前证明，oxlipin 12-oxo-phytodienoic acid（OPDA）的行为沿着脱落酸调节种子萌发拟南芥，但这种协同作用的机制细节仍有待阐明。在这里，我们表明OPDA通过脱落酸、脱落酸敏感ABI 5蛋白和赤霉素敏感RGL 2 DELLA蛋白的发芽抑制作用起作用。我们进一步证明，OPDA也通过另一个休眠促进因子，母亲的FT和TFL 1（MFT）。脱落酸和MFT都通过促进其积累而正向反馈到OPDA途径中。这些结果证实了OPDA在调节拟南芥种子休眠和萌发中的核心作用。拟南芥和强调OPDA和其他休眠促进因子，如脱落酸，RGL 2，和MFT之间的相互作用的复杂性。
We demonstrate that 12-oxo-phytodienoic acid acts together with ABA in a complex regulatory network to promote seed dormancy and repress germination in Arabidopsis. We previously demonstrated that the oxylipin 12-oxo-phytodienoic acid (OPDA) acts along with abscisic acid to regulate seed germination in Arabidopsis thaliana, but the mechanistic details of this synergistic interaction remain to be elucidated. Here, we show that OPDA acts through the germination inhibition effects of abscisic acid, the abscisic acid-sensing ABI5 protein, and the gibberellin-sensing RGL2 DELLA protein. We further demonstrate that OPDA also acts through another dormancy-promoting factor, MOTHER-OF-FT-AND-TFL1 (MFT). Both abscisic acid and MFT positively feed back into the OPDA pathway by promoting its accumulation. These results confirm the central role of OPDA in regulating seed dormancy and germination in A. thaliana and underline the complexity of interactions between OPDA and other dormancy-promoting factors such as abscisic acid, RGL2, and MFT.