Development of insect-resistant transgenic rice with Cry1C*-free endosperm
Development of insect-resistant transgenic rice with Cry1C*-free endosperm
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DOI：
10.1002/ps.1788
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发表时间：
2009-09-01
影响因子：
4.1
通讯作者：
Lin, Yongjun
中科院分区：
文献类型：
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作者：
Ye, Rongjian;Huang, Haiqun;Lin, Yongjun
背景技术背景：三化螟（Tryporyza incertulas步行者）、二化螟（Chilo suppressalis步行者）和稻纵卷叶螟（Cnaphalocrocis medinalis Guenec）是三种严重危害水稻的鳞翅目害虫。在本研究中，新的抗虫转基因水稻，其中Bt蛋白表达几乎没有在胚乳中。将由水稻rbcS启动子（核酮糖-1，5-二磷酸羧化酶/加氧酶小亚基）驱动的抗病基因cry 1C * 导入水稻中花11号（Oryza sativa L.）SSP.结果：共获得83个独立的转化子，其中19个为单拷贝外源基因插入。在田间对这19个转化体的T-1家系进行初步筛选后，筛选出6个高抗虫纯合株系。对这六个纯合转基因品系进行田间测试，以获得对卷叶螟和茎螟的抗性，以及它们的农艺性能。用酶联免疫吸附法（ELISA）测定叶片和胚乳中Cry 1C * 蛋白的含量。获得了抗螟虫、抗稻纵卷叶螟、抗稻曲病、农艺性状正常、胚乳中Cry 1C * 表达量仅为2.6ng g（-1）的转基因水稻RJ 5。(C)2009年化学工业协会
BACKGROUND: Yellow stem borer (Tryporyza incertulas Walker), striped stem borer (Chilo suppressalis Walker) and leaf folder (Cnaphalocrocis medinalis Guenec) are three lepidopteran pests that cause severe damage to rice in many areas of the world. In this study, novel insect-resistant transgenic rice was developed in which Bt protein expression was nearly absent in the endosperm. The resistant gene, cry1C*, driven by the rice rbcS promoter (small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase), was introduced into Zhonghua 11 (Oryza sativa L. ssp. japonica) by Agrobacterium-mediated transformation.RESULTS: A total of 83 independent transformants were obtained, 19 of which were characterised as single-copy foreign gene insertion. After preliminary screening of the T-1 families of these 19 transformants in the field, six highly insect-resistant homozygous lines were selected. These six homozygous transgenic lines were field tested for resistance to leaf folders and stem borers, and for their agronomic performance. The Cry1C* protein levels in leaves and endosperm were measured by ELISA. Subsequently, the elite transgenic line RJ5 was selected; this line not only possessed high resistance to leaf folders and stem borers, normal agronomic performance, but also Cry1C* expression was only 2.6 ng g(-1) in the endosperm.CONCLUSION: These results indicated that RJ5 has the potential for widespread utility in rice production. (C) 2009 Society of Chemical Industry