SENSITIVE AND RAPID ASSAY ON MT-4 CELLS FOR DETECTION OF ANTIVIRAL COMPOUNDS AGAINST THE AIDS VIRUS
SENSITIVE AND RAPID ASSAY ON MT-4 CELLS FOR DETECTION OF ANTIVIRAL COMPOUNDS AGAINST THE AIDS VIRUS
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DOI：
10.1016/0166-0934(87)90002-4
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发表时间：
1987-06-01
影响因子：
3.1
通讯作者：
VANDEPUTTE, M
中科院分区：
文献类型：
--
作者：
PAUWELS, R;DECLERCQ, E;VANDEPUTTE, M
人类免疫缺陷病毒（HIV）感染MT-4细胞和HTLV-I转化的T细胞系被证明是一种快速和灵敏的检测系统，用于检测对获得性免疫缺陷综合征（AIDS）有效的潜在抗病毒药物。MT-4细胞接种HIV后4天，用间接免疫荧光显微镜和激光流式细胞荧光术平行监测病毒抗原表达。当在这些条件下评价3“-叠氮基-2”，3“-双脱氧胸苷（AzddThd，AZT）和2”，3“-双脱氧胞苷（ddCyd）时，它们分别在0.0004和0.02 μ M的最小（33%抑制）浓度下抑制病毒抗原表达。在基于细胞存活率抑制病毒致细胞病变性的试验中，发现AzddThd和ddCyd的最低有效浓度相似。虽然激光流式细胞荧光术可以最好地适用于定量测量，细胞存活和重建破碎的细胞聚集体给出了一个同样快速和灵敏的终点，后者可能是理想的适合初步药物筛选。
Human immunodeficiency virus (HIV) infection of MT-4 cells, and HTLV-I-transformed T-cell line, proved to be a rapid and sensitive assay system for the detection of potential antiviral drugs effective against the acquired immune deficiency syndrome (AIDS). Four days after HIV inoculation of the MT-4 cells, viral antigen expression was monitored in parallel with indirect immunofluorescence microscopy and laser flow cytofluorography. When 3''-azido-2'',3''-dideoxythymidine (AzddThd, AZT) and 2'',3''-dideoxycytidine (ddCyd) were evaluated under these conditions, they inhibited viral antigen expression at a minimum (33% inhibitory) concentration of 0.0004 and 0.02 .mu.M, respectively. Similar minimum effective concentrations were found for AzddThd and ddCyd in assays where inhibition of viral cytopathogenicity was based on cell survival. While laser flow cytofluorography could be best adapted for quantitative measurements, cell survival and reconstitution of disrupted cell aggregates gave an equally rapid and sensitive endpoint; and the latter may be ideally suited for preliminary drug screening.