A Cell Culture System from the Eye Stalk of Penaeus Indicus
A Cell Culture System from the Eye Stalk of Penaeus Indicus
复制标题
DOI：
10.1007/978-94-010-0369-8_62
复制
发表时间：
2001
期刊：
影响因子：
--
通讯作者：
G. Kumar;I. Singh;R. Philip
中科院分区：
文献类型：
--
作者：
G. Kumar;I. Singh;R. Philip
在印度，首次用一种简单的技术从印度对虾的眼柄中培养出细胞体系。反复试验可获得100%的重复性，培养的细胞在等离子瓶中可达到100%的融合。培养培养基为leibbovitz (L-15) 45%, (2X) (HIMEDIA, Bombay)，添加18%胎牛血清（FBS）， 27%对虾肌提取物（PME）， 10%对虾血淋巴（PHL）和由青霉素、硫酸链霉素和氯霉素组成的抗生素混合物。调节培养基pH为6.8 ~ 7.2，渗透压为720±10 mOsm，孵育温度为25℃。在CO2培养箱中，在5% CO2的气氛中，细胞在封闭体系和开放体系中均可培养发育。有趣的是，在所有情况下，细胞增殖和从培养基迁移并形成单层可在24小时内开始，培养物可维持60天以上而不发生液体变化。发育的细胞呈上皮样，有胞内颗粒。
For the first time in India a cell culture system from the eye stalk ofPenaeus indicushas been developed by a simple technique. On repeated trials 100% reproducibility could be obtained and the cell cultures developed could attain 100% confluency in plasdc bottles. The medium used for the development consisted of Leibovitz (L-15) 45%, (2X) (HIMEDIA, Bombay), supplemented with 18% foetal bovine serum (FBS), 27% prawn muscle extract (PME), 10% prawn haemolymph (PHL) and antibiodc mixture consisdng of penicillin, streptomycin sulphate and chloramphenicol. pH of the medium was adjusted to 6.8 to 7.2 and the osmolarity 720 ± 10 mOsm and the temperature of incubadon was 25°C. The cell culture development could be attained in closed system as well as in open system in a CO2incubator in an atmosphere of 5% CO2. Interesdngly in all instances cell proliferadon and migration from the expiants and formation of monolayer could commence within 24 hours and the cultures developed could be maintained for more than 60 days without fluid change. The cells developed were epitheloid like with intracellular granules.