Increased Expression of Acidic Mammalian Chitinase and Chitotriosidase in the Nasal Mucosa of Patients with Allergic Rhinitis
Increased Expression of Acidic Mammalian Chitinase and Chitotriosidase in the Nasal Mucosa of Patients with Allergic Rhinitis
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DOI：
10.1002/lary.20863
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发表时间：
2010-05-01
期刊：
影响因子：
2.6
通讯作者：
Lee, Sang Hag
中科院分区：
文献类型：
--
作者：
Cho, Woo Sung;Kim, Tae Hoon;Lee, Sang Hag
目的/假设：酸性哺乳动物几丁质酶（AMCase）已成为过敏性哮喘动物模型和人类的重要介质。近年来，壳三糖苷酶因其吞噬特异性表达而被认为在天然免疫中发挥作用。因此，AMCase和壳三糖苷酶可能在变应性鼻粘膜的发病机制中起作用。AMCase和壳三糖苷酶的表达和分布模式，因此，确定在正常和过敏性鼻mucosa.Study设计：对照，前瞻性study.Methods：正常下鼻甲粘膜中获得的患者谁被送入隆鼻术。变应性鼻甲骨粘膜取自鼻中隔-鼻甲骨手术期间患有常年性变应性鼻炎的患者。结果：RT-PCR和Western blot检测到AMCase和壳三糖苷酶mRNA和蛋白在正常和变应性鼻黏膜中的表达。与正常鼻甲粘膜相比，过敏性鼻甲粘膜中AMCase和壳三糖苷酶mRNA和蛋白的表达水平均升高。在正常和过敏性鼻甲骨粘膜中，在上皮、炎症细胞和粘膜下腺体中均检测到AMCase和壳三糖酶。AMCase和壳三糖苷酶在变应性鼻黏膜中的表达强度明显高于正常鼻黏膜。结论：AMCase和壳三糖苷酶在正常鼻甲黏膜中组成性表达，提示其参与了对含几丁质病原体的防御。这些几丁质酶在过敏性条件下的上调表明它们可能在鼻过敏反应中起作用，就像过敏性鼻炎中的其他炎症介质一样。
Objectives/Hypothesis: Acidic mammalian chitinase (AMCase) has emerged as an important mediator of allergic asthma in both animal models and in humans. Recently, chitotriosidase has been suggested to play a role in innate immunity because of its phagocytic-specific expression. Thus, AMCase and chitotriosidase may play a role in the pathogenesis of allergic nasal mucosa. The expression and pattern of distribution of AMCase and chitotriosidase were, therefore, determined in normal and allergic nasal mucosa.Study Design: Controlled, prospective study.Methods: Normal inferior turbinate mucosa was obtained in patients who were admitted for augmentation rhinoplasty. Allergic turbinate mucosa was obtained from patients who had perennial allergic rhinitis during septo-turbinate surgery. Reverse transcriptase-polymerase chain reaction (RT-PCR), immunohistochemistry, and Western blotting were applied to the normal and allergic nasal mucosa.Results: The expression of AMCase and chitotriosidase mRNAs and proteins analyzed by RT-PCR and Western blot were detected in all normal and allergic turbinate mucosa tested. The levels of expression of AMCase and chitotriosidase mRNAs and proteins were increased in allergic turbinate mucosa compared with normal turbinate mucosa. In both normal and allergic turbinate mucosa, AMCase and chitotriosidase were detected in the epithelium, inflammatory cells, and submucosal glands. The staining intensity for AMCase and chitotriosidase was stronger in allergic nasal mucosa than normal nasal mucosa.Conclusions: AMCase and chitotriosidase are constitutively expressed in normal turbinate mucosa, suggesting involvement in defense against chitin-containing pathogens. Upregulation of these chitinases in allergic condition suggests that they may play a role in the nasal allergic reaction like other inflammatory mediators in allergic rhinitis.