Identification of an upstream activation sequence and other cis-acting elements required for transcription of COX6 from Saccharomyces cerevisiae.
Identification of an upstream activation sequence and other cis-acting elements required for transcription of COX6 from Saccharomyces cerevisiae.
复制标题
鉴定酿酒酵母中 COX6 转录所需的上游激活序列和其他顺式作用元件。
DOI：
10.1128/mcb.9.12.5350-5358.1989
复制
发表时间：
1989
影响因子：
5.3
通讯作者：
Poyton,RO
中科院分区：
文献类型：
--
作者：
Trawick,JD;Rogness,C;Poyton,RO
酿酒酵母COX6的转录是细胞色素氧化酶VI亚基的核基因，在血红素熟练的细胞中被激活，需要HAP2基因，并受到葡萄糖抑制。在本研究中，通过对C0X6启动子的缺失突变，我们确定了两个对转录重要的区域。第一个是上游激活位点UAS6。它被发现包含在一个84碱基对(BP)的序列中，位于COX6翻译起始密码子的BP-256和-340之间，并且包含被血红素和HAP2激活以及从葡萄糖抑制中释放所需的序列。当该片段位于Cyc1-laclfusion基因的上游时，其两个UASs都被缺失，该片段作为UAS元件发挥作用。尽管UAS6可以促进HAP2和碳源的表达，但它对HAP2和碳源的反应表现出明显的方位依赖性。第二个区域位于BP-255和-91之间。它包含COX6mRNAs的三个主要5‘末端中的两个和一个可能的TATA盒。对该区域的缺失分析表明，假定的TATA盒不是转录所必需的，并且该区域可分为两个冗余结构域。
Transcription ofSaccharomyces cerevisiae COX6, the nuclear gene for subunit VI of cytochromecoxidase, is activated in heme-proficient cells, requires theHAP2gene, and is subject to glucose repression. In this study, by deletion mutagenesis of theC0X6promoter, we identified two regions that are important for transcription. The first was an upstream activation site, UAS6. It was found to be contained within an 84-base-pair (bp) sequence, between bp –256 and –340 of theCOX6translational initiation codon, and to contain sequences required for activation by heme andHAP2and for release from glucose repression. When located upstream of aCyC1-laclfusion gene, deleted for both of its UASs, this segment functioned as a UAS element. Although UAS6could promote expression in either orientation, it showed a marked orientation dependence in its response toHAP2and the carbon source. The second region lay between bp -255 and -91. It contained two of the three major 5′ termini ofCOX6mRNAs and a putative TATA box. Deletion analysis of this region demonstrated that the putative TATA box is not required for transcription and that this region is separable into two redundant domains.