Gingival crevicular interleukin-1 and interleukin-1 receptor antagonist levels in periodontally healthy and diseased sites
Gingival crevicular interleukin-1 and interleukin-1 receptor antagonist levels in periodontally healthy and diseased sites
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DOI：
10.1111/j.1600-0765.1997.tb00568.x
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发表时间：
1997-08-01
影响因子：
3.5
通讯作者：
Noguchi, T
中科院分区：
文献类型：
--
作者：
Ishihara, Y;Nishihara, T;Noguchi, T
白细胞介素-1 （IL-1）分子、IL-1 α和IL-1 β是参与急性期抗感染反应和牙周破坏发病机制的细胞因子。外源性IL-1受体拮抗剂（IL-1ra）可有效减少IL-1介导的炎症反应。然而，这三种天然存在的IL-1分子与牙周病之间的关系尚不清楚。目的探讨龈沟IL-1分子与不同严重程度牙周炎患者临床状况的相关性。对2例健康受试者和7例不同类型牙周炎患者的病变部位的75份龈沟液（GCF）样本进行了IL-1 α、IL-1 β、IL-1ra及IL-1/IL-1ra总比值（IL-1活性指数；IL-1AI）的测定。采用特异性非交叉反应酶联免疫吸附法测定IL-1 α、IL-1 β和IL-1ra。采集GCF时记录探诊深度、牙龈指数及各部位牙槽骨丢失情况。IL-1 α、IL-1 β和IL-1AI总量与牙槽骨丢失评分相关，而IL-1ra总量与牙槽骨丢失评分无关。在牙周炎患者的牙龈组织中也检测到三种IL-1分子。在牙周炎患者的牙龈组织中也检测到类似的IL-1AI的进行性下降。这些结果表明龈沟IL-1和IL-1AI的含量与牙周病的严重程度密切相关。
Interleukin-1 (IL-1) molecules, IL-1 alpha and IL-1 beta are cytokines involved in the acute-phase response against infection and in the pathogenesis of periodontal destruction. Administration of exogenous IL-1 receptor antagonist (IL-1ra) is effective in reducing the inflammatory reactions mediated by IL-1. However, the relationship between these three naturally occurring IL-1 molecules and periodontal diseases has been poorly characterized. We investigated the correlation of gingival crevicular IL-1 molecules and the clinical status of patients with different severities of periodontitis. IL-1 alpha, IL-1 beta, IL-1ra and the total IL-1/IL-1ra ratio (IL-1 activity index; IL-1AI) were measured in 75 gingival crevicular fluid (GCF) samples from non-inflamed gingiva sites in 2 healthy subjects and diseased sites in 7 patients with several types of periodontitis. IL-1 alpha, IL-1 beta and IL-1ra were measured by specific non-crossreactive enzyme linked immunosorbent assay. The probing depth, gingival index and alveolar bone loss of each site was recorded at the time of GCF sampling. The total amount of IL-1 alpha, IL-1 beta and the IL-1AI, but not total IL-1ra, were found to be correlated with alveolar bone loss score. Three IL-1 molecules were also measured in the gingival tissue of patients with periodontitis. A similar progressive decrease of the IL-1AI was detected in gingival tissue with periodontitis. These results suggest that the amounts of both crevicular IL-1 and IL-1AI are closely associated with periodontal disease severity.