Rapid induction of Pavlovian approach to an ethanol-paired visual cue in mice
Rapid induction of Pavlovian approach to an ethanol-paired visual cue in mice
复制标题
DOI：
10.1007/s00213-007-0704-4
复制
发表时间：
2007-06-01
影响因子：
3.4
通讯作者：
Patel, Priya
中科院分区：
文献类型：
--
作者：
Cunningham, Christopher L.;Patel, Priya
虽然许多研究已经证明了巴甫洛夫条件接近与自然刺激配对的线索，但用药物刺激诱导这种行为是相当困难的。本实验测试了一种新的巴甫洛夫方法诱导接近与乙醇配对的条件刺激（CS）。小鼠（NZB/B1NJ，DBA/2 J）在暴露于含有独特视觉线索（星星）的矩形室10分钟之前立即接受乙醇（2 g/kg）的腹膜内注射。一端（配对组，CS+试验）。在隔日，盐水注射之前的设备暴露没有明显的线索（CS试验）。在每次CS+试验后60-75分钟，未配对的对照小鼠在笼中接受乙醇。NZB/B1 NJ配对组小鼠在星星附近花费的时间增加（> 85%的会话），而未配对组小鼠则没有。DBA/2 J配对组小鼠在星星侧花费的时间比未配对组小鼠略多，但没有显示出获取曲线。后处理试验显示，在盐水注射后，两种菌株的配对组中对星星侧有强烈的偏好。然而，只有NZB/B1 NJ小鼠表现出的偏好后ethanol.This研究提供了第一个明确的证明巴甫洛夫条件反射的方法，乙醇配对的视觉刺激的动物的行为和药物暴露之间没有任何偶然性。这种效应在其强度和产生的速度（在2-3次试验中）方面都是显着的，可能与伴随酒精和药物成瘾的线索相关渴望有关。
Although many studies have shown Pavlovian conditioned approach to cues paired with natural reinforcers, it has been quite difficult to induce such behavior with drug reinforcers.This experiment tested a novel Pavlovian procedure for inducing approach to a conditioned stimulus (CS) paired with ethanol.Mice (NZB/B1NJ, DBA/2J) received intraperitoneal injections of ethanol (2 g/kg) immediately before 10-min exposure to a rectangular chamber that contained a distinctive visual cue (star) at one end (Paired group, CS+ trials). On alternate days, saline injection preceded apparatus exposure with no distinctive cues (CS- trials). Unpaired control mice received ethanol in the home cage 60-75 min after each CS+ trial.NZB/B1NJ Paired group mice spent increasing amounts of time (> 85% of the session) in proximity to the star, whereas Unpaired group mice did not. DBA/2J Paired group mice spent slightly more time on the star side than Unpaired group mice but did not show an acquisition curve. Postconditioning tests showed a strong preference for the star side in Paired groups from both strains after saline injection. However, only NZB/B1NJ mice showed a preference after ethanol.This study provides the first unambiguous demonstration of Pavlovian conditioned approach to an ethanol-paired visual stimulus in the absence of any contingency between the animal's behavior and drug exposure. This effect, which is remarkable both in terms of its magnitude and the rapidity with which it was produced (within 2-3 trials), may be related to the cue-associated craving that accompanies alcohol and drug addiction.