[Biological activity of adriamycin in vitro].
[Biological activity of adriamycin in vitro].
复制标题
阿霉素的体外生物活性[J].
作者：
R. Silvestrini;C. Gambarucci;T. Dasdia
阿霉素是一种抗生素，从peucetius链霉菌的突变体中分离出来，具有与道诺霉素非常相似的化学结构，但在实验性肿瘤中具有更高的治疗指数。体外研究了该抗生素在HeLa细胞株上的生物活性。阿霉素迅速渗透到细胞中，并在核周染色质水平上以明显的定位固定在核结构上。它引起有丝分裂过程的明显和直接的干扰，即低剂量时的前期抑制和高剂量时的有丝分裂阻断。甚至DNA和RNA的合成，放射自显像评价为3h -胸腺嘧啶和3h -尿苷的掺入，也明显受到抑制。细胞的生存能力，在产生菌落的能力和细胞群的增殖活性方面进行了测试，严重下降，其程度与处理时间和抗药浓度成正比。
Adriamycin is an antibiotic, isolated from cultures of a mutant of Streptomyces peucetius, var. caesius, with a chemical structure very similar to daunomycin but with a higher therapeutic index in experimental tumors. The biological activity of this antibiotic has been studied in vitro on the HeLa cell strain. Adriamycin quickly penetrates into the cells and fixes to the nuclear structures with a marked localization at the level of the perinucleolar chromatin. It causes a marked and immediate disturbance of the mitotic process, viz. pre-prophasic inhibition at the low doses and mitotic block at the higher doses. Even the synthesis of DNA and RNA, evaluated autoradiographically as incorporation of 3H-thymidine and 3H-uridine, appear markedly inhibited. The viability of the cells, tested both as regards capacity to give rise to colonies and as regards proliferative activity of a cell population, was seriously reduced, in a degree proportional to the period of treatment and to the concentration of the antibi...