Genetically induced cell death in bulge stem cells reveals their redundancy for hair and epidermal regeneration.
Genetically induced cell death in bulge stem cells reveals their redundancy for hair and epidermal regeneration.
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DOI：
10.1002/stem.1910
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发表时间：
2015-03
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影响因子：
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通讯作者：
Frye M
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文献类型：
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作者：
Driskell I;Oeztuerk-Winder F;Humphreys P;Frye M
成年哺乳动物表皮含有多种干细胞群，其中静止的干细胞群和增殖性更强的干细胞群和祖细胞群共存。然而，这些群体在体内平衡中的精确相互关系仍不清楚。在这里，我们通过对维持成人皮肤所需的必需组蛋白甲基转移酶 Setd8 进行基因消融，阻断了表达静态角蛋白 19 (K19) 的凸起干细胞对毛囊形成的贡献。 Setd8的缺失通过抑制细胞分裂和诱导细胞死亡消除了凸起细胞对毛囊再生的贡献，但毛囊的生长和形态不受影响。此外，去除毛囊凸起中的 Setd8 可以阻断 K19 阳性干细胞对受伤表皮的贡献，但伤口愈合过程没有改变。我们的数据表明，短期内静止的凸出干细胞对于毛囊再生和表皮损伤来说是可有可无的，并支持静止和循环干细胞群等效的假设。干细胞 2015；33：988–998
Adult mammalian epidermis contains multiple stem cell populations in which quiescent and more proliferative stem and progenitor populations coexist. However, the precise interrelation of these populations in homeostasis remains unclear. Here, we blocked the contribution of quiescent keratin 19 (K19)-expressing bulge stem cells to hair follicle formation through genetic ablation of the essential histone methyltransferase Setd8 that is required for the maintenance of adult skin. Deletion of Setd8 eliminated the contribution of bulge cells to hair follicle regeneration through inhibition of cell division and induction of cell death, but the growth and morphology of hair follicles were unaffected. Furthermore, ablation of Setd8 in the hair follicle bulge blocked the contribution of K19-postive stem cells to wounded epidermis, but the wound healing process was unaltered. Our data indicate that quiescent bulge stem cells are dispensable for hair follicle regeneration and epidermal injury in the short term and support the hypothesis that quiescent and cycling stem cell populations are equipotent. Stem Cells 2015;33:988–998