Lignans from Patrinia scabiosaefolia improve insulin resistance by activating PI-3K/AKT pathway and promoting GLUT4 expression
Lignans from Patrinia scabiosaefolia improve insulin resistance by activating PI-3K/AKT pathway and promoting GLUT4 expression
复制标题
败酱木脂素通过激活 PI-3K/AKT 通路并促进 GLUT4 表达改善胰岛素抵抗
DOI：
10.1016/j.fshw.2023.03.015
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发表时间：
2023-11-01
影响因子：
7
通讯作者：
Kang，Wenyi
中科院分区：
文献类型：
--
作者：
Liu，Zhenhua;Wang，Mengke;Kang，Wenyi
黄花败菜（Patrinia scabiosaefolia），作为野生蔬菜在中国已有2000多年的历史，具有多种药理活性，包括抗炎、抗肿瘤和降血糖。基于我们正在进行的对p的化学成分和降糖活性的研究。分离鉴定了4个木脂素化合物，分别为(+)-异核桃树脂醇(1)、7R、7'R、8S、8S -(+)-新橄榄-4- o- β- d -葡萄糖吡喃苷(2)、4- o-甲基雪松素(3)和帕特利安A(4)。降糖活性表明，化合物2和3在100µmol/L （P< 0.001）、50µmol/L （P< 0.001）和25µmol/L （P< 0.01）时能极显著改善IR 3T3-L1细胞的胰岛素抵抗。而化合物4仅在100µmol/L时促进IR 3T3-L1细胞对葡萄糖的摄取（P< 0.01）。Western blotting实验表明，化合物2和4上调p-IRS、PI-3K、p-AKT和葡萄糖转运蛋白4 （GLUT4）的蛋白表达，促进glut4mrna的转录。因此，我们推测化合物2和4通过激活PI-3K/AKT信号通路来改善IR。
Patrinia scabiosaefolia, is used as wild vegetable in China for more than 2 000 years, with a variety of pharmacological activities, including anti-inflammatory, anti-tumor and hypoglycemic. Based on our ongoing research on chemical constituents and hypoglycemic activity ofP. scabiosaefolia, 4 lignan compounds, (+)-isolariciresinol (1), 7R,7'R,8S,8'S-(+)-neo-olivil-4-O-β-D-glucopyranoside (2), 4-O-methylcedrusin (3) and patrinian A (4), were isolated and identified. The hypoglycemic activity showed that compounds2and3could extremely significantly improve insulin resistance at 100 (P< 0.001), 50 (P< 0.001) and 25 µmol/L (P< 0.01) in IR 3T3-L1 cells. While compound4only promoted glucose uptake by IR 3T3-L1 cells at 100 µmol/L (P< 0.01). Western blotting experiments showed that compounds2and4up-regulated the protein expressions of p-IRS, PI-3K, p-AKT and glucose transporter 4 (GLUT4), and promoted the transcription ofGLUT4mRNA. Therefore, the mechanisms of compounds2and4were presumed to improve IR by activating PI-3K/AKT signaling pathway.