Genome-wide analysis of gene expression in human embryonic tooth germ
Genome-wide analysis of gene expression in human embryonic tooth germ
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人类胚胎牙胚基因表达的全基因组分析
DOI：
10.1007/s10735-014-9580-5
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发表时间：
2014-12-01
影响因子：
3.2
通讯作者：
Zhang, Yanding
中科院分区：
文献类型：
--
作者：
Huang, Zhen;Hu, Xuefeng;Zhang, Yanding
虽然许多基因在小鼠牙齿发育过程中起着重要的调控作用，但对人类牙齿发育过程中的基因表达谱及其功能知之甚少。为了揭示人类牙源性基因的表达谱，我们利用基因芯片技术分析了11周龄人胎儿牙胚和唇组织中基因表达的差异。我们确定了167个基因，强烈表达的帽状期牙胚相比，唇组织。其中，通过基因本体富集分析进一步鉴定了145个基因，这些基因在多个基因本体类别中具有高度代表性，包括细胞外成分、序列特异性DNA结合蛋白、Wnt蛋白结合分子、系统发育、器官发生和细胞分化。67个已知与哺乳动物牙齿发育和牙齿异常相关的基因被鉴定出来。实时PCR进一步用于验证微阵列数据。原位杂交结果显示ISL 1和BARX 1在切牙、尖牙和磨牙中的表达具有牙型特异性，与基因芯片结果一致。我们的研究结果代表了一组可靠的数据，可以为未来阐述人类牙齿发育的分子机制提供坚实的基础。
While numerous genes that play important regulatory roles during tooth development in mice have been identified, little is known about gene expression profile and their function during human odontogenesis. To unveil expression profile of odontogenic genes in humans, we conducted genome-wide gene expression analysis by microarray assays to analyze differential gene expression between tooth germ and lip tissue from 11-week old human fetuses. We identified 167 genes that are strongly expressed in the cap stage tooth germ as compared to the lip tissue. Among them, 145 genes were further identified by gene ontology enrichment analysis that are highly represented in multiple gene ontology classes, include extracellular components, sequence-specific DNA binding proteins, Wnt-protein binding molecules, system development, organogenesis, and cell differentiation. Sixty-seven genes that are known to be associated with mammalian tooth development and tooth abnormalities were identified. Real-time PCR was further employed to validate microarray data. Moreover, in situ hybridization assay demonstrated tooth type specific expression ofISL1andBARX1in the incisor, canine, and molar respectively, consistent with microarray results. Our results represent a set of reliable data that could provide a solid base for future elaboration of molecular mechanisms underlying human tooth development.