HLA-DR ALLELES IN PATIENTS WITH SJÖGREN'S SYNDROME OVER-REPRESENTING Vβ2 AND Vβ13 GENES IN THE LABIAL SALIVARY GLANDS
HLA-DR ALLELES IN PATIENTS WITH SJÖGREN'S SYNDROME OVER-REPRESENTING Vβ2 AND Vβ13 GENES IN THE LABIAL SALIVARY GLANDS
复制标题
干燥综合征患者的 HLA-DR 等位基因在唇唾液腺中过度表达 Vβ2 和 Vβ13 基因
DOI：
10.1093/rheumatology/33.5.420
复制
发表时间：
1994
期刊：
影响因子：
5.5
通讯作者：
S. Yoshida
中科院分区：
文献类型：
--
作者：
T. Sumida;T. Sakamaki;F. Yonaha;T. Maeda;T. Namekawa;Y. Nawata;K. Takabayashi;I. Iwamoto;S. Yoshida
为探讨唇部V β 2和V β 13基因过表达的SS患者发病机制中的遗传因素，采用聚合酶链反应（PCR）和序列特异性寡核苷酸探针对10例唇部V β 2和V β 13基因显性表达的SS患者HLA-DR和-DQ等位基因进行分析。用PCR方法测定了V β 2和V β 13基因cDNA克隆的CDR3氨基酸序列。结果显示，与健康人群（分别为35.6%和26%）相比，DRB4*0101等位基因频率显著升高（80%），DRB3等位基因频率显著降低（0%）。测序分析表明，75%的V β 2 cDNA克隆和87%的V β 13 cDNA克隆在CDR13区106位有谷氨酰胺残基。此外，在两例SS患者的两个不同克隆（27%）中检测到V β 13阳性T细胞CDR3中的保守序列（Y*TLRNEQ）。这些结果提示，DRB3基因的减少和DRB4*0101基因的增加可能与V β 2-和V β 13阳性T细胞识别的抗原有关。
: To identify genetic factors that play a role in the pathogenesis of patients with SS over-representing V beta 2 and V beta 13 genes in the lips, HLA-DR and -DQ alleles of 10 primary SS patients with predominant expression of V beta 2 and V beta 13 genes in the lips were analysed, using the polymerase chain reaction (PCR) and sequence specific oligonucleotide probes. The CDR3 amino acid sequences of cDNA clones encoding V beta 2 and V beta 13 genes were also determined by PCR. The results showed that the DRB4*0101 allele was significantly increased (80%) and that the frequency of DRB3 allele was decreased (0%) when compared to findings in healthy subjects (35.6 and 26%, respectively). Sequencing analyses demonstrated that 75% of V beta 2 cDNA clones and 87% of V beta 13 cDNA clones had a glutamine residue at position 106, in the CDR13 region. Moreover, the conserved sequences (Y*TLRNEQ) in the CDR3 of V beta 13-positive T cell were detected in two different clones (27%) from the two individual SS patients. These findings suggest that the decreased DRB3 and increased DRB4*0101 alleles may be associated with the antigens recognized by V beta 2- and V beta 13-positive T cells.