Kinetics of cytokines mRNAs expression in the dorsal skin of WBN/ILA-Ht rats following topical application of T-2 toxin
Kinetics of cytokines mRNAs expression in the dorsal skin of WBN/ILA-Ht rats following topical application of T-2 toxin
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DOI：
10.1078/0940-2993-00189
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发表时间：
2001-09-01
影响因子：
--
通讯作者：
Doi, K
中科院分区：
文献类型：
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作者：
Albarenque, SM;Suzuki, K;Doi, K
在局部应用T-2毒素的Wistar衍生的低血糖WBN/ILA-Ht大鼠的背部皮肤中检查细胞因子mRNA表达的动力学。在施用10穆尔（0.5 μ g/穆尔）T-2毒素溶液后，在处理后3、6、12和24小时（HAT）从皮肤活组织检查中获得总mRNA。用对应于大鼠TNF-α、IL-1 α、IL-1 β、IL-6和IL-10细胞因子的cDNA序列的寡核苷酸引物对进行逆转录-聚合酶链反应（RT-PCR）。TNF-α mRNA水平在3 HAT时显著升高，向24 HAT时下降，但即使在24 HAT时仍保持显著高水平。此外，IL-1 β mRNA表达水平在3和24 HAT时显示轻微但显著的升高。另一方面，在观察期内，在T-2毒素处理组和对照组之间没有观察到其他细胞因子mRNA表达的显著差异。结合我们先前描述表皮细胞凋亡序列的报道（ALBARENQUE等，1999），本研究结果表明TNF-α mRNA表达的升高可能在T-2毒素诱导的表皮细胞凋亡中起重要作用。
The kinetics of cytokines mRNAs expression was examined in the dorsal skin of Wistar-derived hypotrichotic WBN/ILA-Ht rats topically applied with T-2 toxin. After the application of 10 mul (0.5 mug/mul) of T-2 toxin solution, the total mRNA was obtained from skin biopsies at 3, 6, 12 and 24 hours after treatment (HAT). Reverse transcription-polymerase chain reaction (RT-PCR) was carried out with pairs of oligonucleotide primers corresponding to the cDNA sequences of rat TNF-alpha, IL-1 alpha, IL-1 beta, IL-6 and IL-10 cytokines. The level of TNF-alpha mRNA showed marked elevation at 3HATand decreased toward 24HAT, but it remained significantly higher level even at 24HAT. In addition, the level of IL-1 beta mRNA expression showed a sligth but significant elevation at 3 and 24HAT. On the other hand, no significant differences were observed in other cytokines mRNAs expression between T-2 toxin-treated and control groups througth the observation period. Together with our previous report describing the sequence of epidermal cell apoptosis (ALBARENQUE et al. 1999), the present results suggest that the elevation of TNF-a mRNA expression may play an important role in T-2 toxin-induced epidermal cell apoptosis.