A glycine betaine importer limits Salmonella stress resistance and tissue colonization by reducing trehalose production.
A glycine betaine importer limits Salmonella stress resistance and tissue colonization by reducing trehalose production.
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DOI：
10.1111/j.1365-2958.2012.08022.x
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发表时间：
2012-04
影响因子：
3.6
通讯作者：
Detweiler CS
中科院分区：
文献类型：
--
作者：
Pilonieta MC;Nagy TA;Jorgensen DR;Detweiler CS
Mechanisms by which Salmonella establish chronic infections are not well understood. Microbes respond to stress by importing or producing compatible solutes, small molecules that stabilize proteins and lipids. The Salmonella locus opuABCD (also called OpuC) encodes a predicted importer of the compatible solute glycine betaine. Under stress conditions, if glycine betaine cannot be imported, S. enterica produce the disaccharide trehalose, a highly effective compatible solute. We demonstrate that strains lacking opuABCD accumulate more trehalose under stress conditions than wild-type strains. ΔopuABCD mutant strains are more resistant to high salt, low pH and hydrogen peroxide, conditions that mimic aspects of innate immunity, in a trehalose-dependent manner. In addition, ΔopuABCD mutant strains require the trehalose production genes to out-compete wild-type strains in mice and macrophages. These data suggest that in the absence of opuABCD, trehalose accumulation increases bacterial resistance to stress in broth and mice. Thus, opuABCD reduces bacterial colonization via a mechanism that limits trehalose production. Mechanisms by which microbes limit disease may reveal novel pathways as therapeutic targets.
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影响因子：
3.2
作者：
Fang, FC;Chen, CY;Xu, YS
通讯作者：
Xu, YS
影响因子：
3.2
作者：
Baek, Chang-Ho;Wang, Shifeng;Curtiss, Roy, III
通讯作者：
Curtiss, Roy, III
影响因子：
6.7
作者：
Gal-Mor O;Gibson DL;Baluta D;Vallance BA;Finlay BB
通讯作者：
Finlay BB
影响因子：
3.6
作者：
Domínguez-Bernal, G;Pucciarelli, MG;García-del Portillo, F
通讯作者：
García-del Portillo, F
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL