Modulation of RNA metal binding by flanking bases: 15N NMR evaluation of GC, tandem GU, and tandem GA sites.
Modulation of RNA metal binding by flanking bases: 15N NMR evaluation of GC, tandem GU, and tandem GA sites.
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通过侧翼碱基调节 RNA 金属结合：GC、串联 GU 和串联 GA 位点的 15N NMR 评估。
DOI：
10.1080/15257770903044234
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Jones,RogerA
中科院分区：
文献类型：
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作者：
Wang,Weimin;Zhao,Jianwei;Han,Qianwei;Wang,Gang;Yang,Gengcheng;Shallop,AnthonyJ;Liu,Jane;Gaffney,BarbaraL;Jones,RogerA
在Mg（H2O）62+、Zn 2+、Cd 2+和Co（NH3）63+存在下的15 N NMR化学位移变化被用来探测侧翼碱基对三种不同RNA基序中金属结合位点的影响。我们发现：对于GC对，侧翼嘌呤的存在仅为软金属Zn 2+和Cd 2+创建了一个位点; GG·UU基序仅选择性地结合Co（NH3）63+，而UG·GU基序不结合这些金属;剪切的GA·AG对的腺苷侧翼的3′鸟苷创建了一个异常强的结合位点，排除了与串联对中交叉链堆叠的鸟苷的结合。
15N NMR chemical shift changes in the presence of Mg(H2O)62+, Zn2+, Cd2+, and Co(NH3)63+were used to probe the effect of flanking bases on metal binding sites in three different RNA motifs. We found that: for GC pairs, the presence of a flanking purine creates a site for the soft metals Zn2+and Cd2+only; a GG·UU motif selectively binds only Co(NH3)63+, while a UG·GU motif binds none of these metals; a 3′ guanosine flanking the adenosine of a sheared GA·AG pair creates an unusually strong binding site that precludes binding to the cross-strand stacked guanosines within the tandem pair.