Enzyme-free amperometric sensing of glucose by using gold nanoparticles
Enzyme-free amperometric sensing of glucose by using gold nanoparticles
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DOI：
10.1002/chem.200501051
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发表时间：
2006-03-20
影响因子：
4.3
通讯作者：
Raj, CR
中科院分区：
文献类型：
--
作者：
Jena, BK;Raj, CR
描述了一种非酶电化学方法，该方法使用在溶胶-凝胶过程获得的三维 (3D) 硅酸盐网络上自组装的金 (Au) 纳米颗粒来检测葡萄糖。纳米级金颗粒在硅酸盐网络的硫醇尾基上自组装，并通过羟胺放大。在没有任何酶或氧化还原介体的情况下，Au 纳米粒子在磷酸盐缓冲溶液（pH 9.2）中以较低的正电势（0.16 V）有效催化葡萄糖的氧化。金纳米粒子修饰传感器 (MPTS-nAuE) 成功用于葡萄糖的电流传感，并且表现出优异的灵敏度，检测限为 50 nm。常见的干扰剂抗坏血酸（AA）不会干扰葡萄糖的检测。 MPTS-nAuE 传感器显示出葡萄糖和 AA 的单独伏安响应。该传感器对0-8 mm范围内的葡萄糖呈线性响应，传感器的灵敏度为0.179 nA cm(-2) nm(-1)。该传感器具有出色的再现性、长期存储和操作稳定性。
A nonenzymatic electrochemical method is described for the detection of glucose by using gold (Au) nanoparticles self-assembled on a three-dimensional (3D) silicate network obtained by using sol-gel processes. The nanosized Au particles have been self-assembled on the thiol tail groups of the silicate network and enlarged by hydroxylamine. The Au nanoparticles efficiently catalyze the oxidation of glucose at less-positive potential (0.16 V) in phosphate buffer solution (pH 9.2) in the absence of any enzymes or redox mediators. The Au nanoparticle-modified transducer (MPTS-nAuE) was successfully used for the amperometric sensing of glucose and it showed excellent sensitivity with a detection limit of 50 nm. The common interfering agent ascorbate (AA) does not interfere with the detection of glucose. The MPTS-nAuE transducer showed individual voltammetric responses for glucose and AA. This transducer responded linearly to glucose in the range of 0-8 mm and the sensitivity of the transducer was found to be 0.179 nA cm(-2) nm(-1). Excellent reproducibility, and long-term storage and operational stability was observed for this transducer.