Co-expression of Anoctamins in Cilia of Olfactory Sensory Neurons
Co-expression of Anoctamins in Cilia of Olfactory Sensory Neurons
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DOI：
10.1093/chemse/bju061
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发表时间：
2015-02-01
期刊：
影响因子：
3.5
通讯作者：
Neuhaus, Eva M.
中科院分区：
文献类型：
--
作者：
Henkel, Bastian;Drose, Daniela R.;Neuhaus, Eva M.
脊椎动物可以感知和识别大量的化学信号。参与化学检测和转导的分子机制在嗅觉感觉神经元的纤毛内表达。目前，只有有限的信息可用于个别信号成分的分布和密度的睫状室内。使用超分辨率显微镜，我们在这里表明，环核苷酸门控通道和钙激活的anoctamin家族的氯离子通道被定位到睫状体膜中的离散微区。除了ANO2之外，第二种抗辛胺ANO6也定位于纤毛微区。这一观察结果以及ANO6和ANO2共定位的事实表明ANO6在嗅觉信号传导中的作用。我们发现，ANO2和ANO6都可以形成异多聚体，这种异聚化改变了重组通道的生理特性。因此，我们提供的证据，相互作用的ANO2和ANO6的嗅觉纤毛，嗅觉信号可能的生理相关性。
Vertebrates can sense and identify a vast array of chemical cues. The molecular machinery involved in chemodetection and transduction is expressed within the cilia of olfactory sensory neurons. Currently, there is only limited information available on the distribution and density of individual signaling components within the ciliary compartment. Using super-resolution microscopy, we show here that cyclic-nucleotide-gated channels and calcium-activated chloride channels of the anoctamin family are localized to discrete microdomains in the ciliary membrane. In addition to ANO2, a second anoctamin, ANO6, also localizes to ciliary microdomains. This observation, together with the fact that ANO6 and ANO2 co-localize, indicates a role for ANO6 in olfactory signaling. We show that both ANO2 and ANO6 can form heteromultimers and that this heteromerization alters the recombinant channels' physiological properties. Thus, we provide evidence for interaction of ANO2 and ANO6 in olfactory cilia, with possible physiological relevance for olfactory signaling.