Age dependent aneuploidy and telomere length of the human vascular endothelium
Age dependent aneuploidy and telomere length of the human vascular endothelium
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DOI：
10.1016/s0021-9150(01)00506-8
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发表时间：
2001-12-01
期刊：
影响因子：
5.3
通讯作者：
Aviv, A
中科院分区：
文献类型：
--
作者：
Aviv, H;Khan, MY;Aviv, A
基本原理：非整倍体和端粒长度是与体外细胞衰老相关的两个主要参数。为了探讨非整倍体和端粒长度在人类脉管系统衰老中的作用，我们在直接制备人腹主动脉内皮细胞时研究了这两个参数。方法：在“触摸准备”载玻片上使用荧光原位杂交，我们评估了腹主动脉非培养内皮细胞中两个常染色体（染色体 6 和 16）和性染色体的非整倍性，作为供体年龄的函数。结果：我们发现血管内皮细胞非整倍体的频率随着年龄的增长而显着增加。这通过年龄依赖性四体性（6 号染色体 r(s) = 0.56，P = 0.006；16 号染色体 r(s) = 0.54，P = 0.008）和年龄依赖性 Y 染色体缺失（r(s) = 0.85，P = 0.0003）来表达。此外，我们发现端粒长度与年龄呈负相关（r = -0.38，P = 0.008）。数据解释：这些发现表明，早期在体外观察到的细胞衰老指标也在人类血管内皮中表达。体内。因此，非整倍性和端粒磨损可能在人类脉管系统的衰老中发挥作用。 (C) 2001 Elsevier Science Ireland Ltd. 保留所有权利。
Rationale: aneuploidy and telomere length are two major parameters that have been associated with cellular senescence in vitro. In order to explore the role of aneuploidy and telomere length in aging of the human vasculature, we studied these two parameters in direct preparations of endothelial cells of the human abdominal aorta. Methods: using fluorescent in situ hybridization on 'touch prep' slides, we evaluated aneuploidy of two autosomes (chromosomes 6 and 16) and sex chromosomes in non cultured endothelial cells of the abdominal aorta as a function of the donor's age. Results: we found that the frequency of aneuploidy of vascular endothelial cells significantly increased with age. This was expressed by age-dependent tetrasomy (r(s) = 0.56, P = 0.006 for chromosome 6; and r(s) = 0.54, P = 0.008 for chromosome 16), and age dependent loss of the Y chromosome (r(s) = 0.85, P = 0.0003). In addition, we found that telomere length was inversely correlated with age (r = -0.38, P = 0.008). Data interpretation: these findings suggest that indicators of cellular senescence, earlier observed in vitro, are also expressed in the human vascular endothelium. in vivo. Aneuploidy and telomere attrition might thus play a role in the aging of the human vasculature. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.