An immunohistochemical study of neuropeptides and neuronal cytoskeletal proteins in the neuroepithelial component of a spontaneous murine ovarian teratoma. Primitive neuroepithelium displays immunoreactivity for neuropeptides and neuron-associated beta-tubulin isotype.
An immunohistochemical study of neuropeptides and neuronal cytoskeletal proteins in the neuroepithelial component of a spontaneous murine ovarian teratoma. Primitive neuroepithelium displays immunoreactivity for neuropeptides and neuron-associated beta-tubulin isotype.
复制标题
DOI：
--
复制
发表时间：
1989-11
期刊：
影响因子：
--
通讯作者：
D. Caccamo;Mary M. Herman;A. Frankfurter;Christos D. Katsetos;V. Collins;L. J. Rubinstein
中科院分区：
文献类型：
--
作者：
D. Caccamo;Mary M. Herman;A. Frankfurter;Christos D. Katsetos;V. Collins;L. J. Rubinstein
大约三分之一的LTXBO品系雌性小鼠发生自发性卵巢畸胎瘤。这些肿瘤含有大量神经上皮成分，包括类似胚胎神经管（髓上皮细胞）的原始神经结构、室管膜母细胞和室管膜细胞、成神经细胞、成熟神经节神经元、有髓鞘神经突和星形胶质细胞。本研究的目的是根据一些特征良好的营养和调节神经肽和神经递质，几个神经元相关的细胞骨架蛋白，和其他蛋白质指示神经元和神经胶质分化的免疫组织化学定位来表征这些肿瘤。髓上皮细胞roepithelium表现出局灶性的类胡萝卜素样、阿片肽样和γ-氨基丁酸样免疫反应性，并显示神经元相关III类β-微管蛋白同种型的免疫染色。成熟的神经节细胞也对这些标记物有免疫反应，此外，对生长抑素、胆囊收缩素、铃蟾肽、胰高血糖素、血管活性肠肽和神经肽Y也有免疫反应。成熟的神经节细胞也与神经元细胞骨架（包括微管相关蛋白，MAP 2和tau蛋白，和更高分子量的磷酸化和非磷酸化的神经丝亚基），神经元特异性烯醇化酶，和突触素相关的蛋白质的免疫反应。未分化的干细胞，室管膜母细胞和室管膜细胞和星形胶质细胞都用单克隆抗体染色，该单克隆抗体识别所有哺乳动物β-微管蛋白同种型，但不与神经元相关的细胞骨架蛋白或神经肽的抗体反应。神经肽样免疫反应性和III类β-微管蛋白同种型的证明表明肿瘤性原始神经上皮中的早期神经元定型。这些模式的免疫反应性密切遵循在哺乳动物和鸟类前脑的正常神经细胞发生中遇到的，并增加的精度，其中进行性神经上皮细胞分化的早期阶段，可以分析在人类胚胎肿瘤的中枢神经系统。
Approximately one third of the female mice of the LTXBO strain develop spontaneous ovarian teratomas. These tumors contain a large neuroepithelial component, which includes primitive neural structures resembling embryonic neural tubes (medulloepithelial rosettes), ependymoblastic and ependymal rosettes, neuroblasts, mature ganglionic neurons, myelinated neurites, and astrocytes. The purpose of this study was to characterize these tumors according to the immunohistochemical location of some well-characterized trophic and regulatory neuropeptides and neurotransmitters, several neuronal-associated cytoskeletal proteins, and other proteins indicative of neuronal and glial differentiation. Medulloepithelial rosettes showed focal serotonin-like, opioid peptide-like and gamma-amino butyric acid-like immunoreactivity, and displayed immunostaining for the neuron-associated class III beta-tubulin isotype. The mature ganglion cells were also immunoreactive for these markers, and, in addition, for somatostatin, cholecystokinin, bombesin, glucagon, vasoactive intestinal peptide, and neuropeptide Y. Mature ganglion cells were also immunoreactive for proteins associated with the neuronal cytoskeleton (including microtubule-associated proteins, MAP2 and tau, and higher molecular weight phosphorylated and non-phosphorylated neurofilament subunits), neuron-specific enolase, and synaptophysin. Undifferentiated stem cells, ependymoblastic and ependymal rosettes, and astroglia all stained with a monoclonal antibody that recognizes all mammalian beta-tubulin isotypes, but did not react with antibodies to neuronal-associated cytoskeletal proteins or neuropeptides. Neuropeptide-like immunoreactivity and demonstration of the class III beta-tubulin isotype indicate early neuronal commitment in neoplastic primitive neuroepithelium. These patterns of immunoreactivity closely follow those encountered in the normal neurocytogenesis of the mammalian and avian forebrain, and increase the precision with which the early stages of progressive neuroepithelial differentiation can be analyzed in human embryonal tumors of the CNS.