Stress responses to polycyclic aromatic hydrocarbons in Arabidopsis include growth inhibition and hypersensitive response-like symptoms
Stress responses to polycyclic aromatic hydrocarbons in Arabidopsis include growth inhibition and hypersensitive response-like symptoms
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DOI：
10.1093/jxb/eri295
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发表时间：
2005-11-01
影响因子：
6.9
通讯作者：
Colón-Carmona, A
中科院分区：
文献类型：
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作者：
Alkio, M;Tabuchi, TM;Colón-Carmona, A
多环芳烃（PAHs）是全球环境问题，因为它们会导致许多健康问题，包括癌症和人体组织炎症。植物在去除大气中的多环芳烃方面起着重要作用，然而，有关植物中多环芳烃胁迫反应的生理学、细胞和分子生物学以及生物化学方面的信息却很缺乏。研究了暴露于三环芳香族化合物菲的拟南芥（Arabidopsis thaliana）中的PAH胁迫反应。PAH胁迫的形态学症状表现为根和地上部生长减少、毛状体变形、根毛减少、萎黄、开花延迟和出现白色斑点，随后发展为坏死性病变。在组织和细胞水平上，植物经历了氧化应激。这通过分别使用3，3 '-二氨基联苯胺和台盼蓝染色检测的局部H2 O2产生和细胞死亡来指示。气相色谱-质谱和荧光光谱分析表明，菲被植物内化。细胞壁疏松蛋白expansin的基因表达被抑制，而致病相关蛋白PR 1的基因表达被诱导响应PAH暴露。这些研究结果表明，（i）拟南芥需要菲，表明可能在植物中降解，（ii）在植物和动物中的多环芳烃反应可能共享类似的应激机制，因为在动物细胞中的多环芳烃解毒也导致氧化应激，和（iii）植物特异性防御机制有助于拟南芥中的多环芳烃应激反应。
Polycyclic aromatic hydrocarbons (PAHs) are of global environmental concern because they cause many health problems including cancer and inflammation of tissue in humans. Plants are important in removing PAHs from the atmosphere; yet, information on the physiology, cell and molecular biology, and biochemistry of PAH stress responses in plants is lacking. The PAH stress response was studied in Arabidopsis (Arabidopsis thaliana) exposed to the three-ring aromatic compound, phenanthrene. Morphological symptoms of PAH stress were growth reduction of the root and shoot, deformed trichomes, reduced root hairs, chlorosis, late flowering, and the appearance of white spots, which later developed into necrotic lesions. At the tissue and cellular levels, plants experienced oxidative stress. This was indicated by localized H2O2 production and cell death, which were detected using 3, 3'-diaminobenzidine and trypan blue staining, respectively. Gas chromatography-mass spectrometry and fluorescence spectrometry analyses showed that phenanthrene is internalized by the plant. Gene expression of the cell wall-loosening protein expansin was repressed, whereas gene expression of the pathogenesis related protein PR1 was induced in response to PAH exposure. These findings show that (i) Arabidopsis takes up phenanthrene, suggesting possible degradation in plants, (ii) a PAH response in plants and animals may share similar stress mechanisms, since in animal cells detoxification of PAHs also results in oxidative stress, and (iii) plant specific defence mechanisms contribute to PAH stress response in Arabidopsis.