Use of antibiotics to prevent hepatitis and typhlitis in male scid mice spontaneously infected with Helicobacter hepaticus.
Use of antibiotics to prevent hepatitis and typhlitis in male scid mice spontaneously infected with Helicobacter hepaticus.
复制标题
DOI：
--
复制
发表时间：
1995-08
期刊：
影响因子：
--
通讯作者：
R. Russell;D. Haines;M. Anver;J. Battles;P. Gorelick;L. L. Blumenauer-L.;Gonda;Jerrold M. Ward
中科院分区：
文献类型：
--
作者：
R. Russell;D. Haines;M. Anver;J. Battles;P. Gorelick;L. L. Blumenauer-L.;Gonda;Jerrold M. Ward
在雄性scid/NCr小鼠中评价了抗生素组合预防急性和进行性慢性肝炎以及与肝螺杆菌感染相关的增殖性盲肠炎的使用。使用的药物组合为阿莫西林-甲硝唑-铋剂、四环素-甲硝唑-铋剂、阿莫西林-新霉素、新霉素单药和阿莫西林单药。从4周龄开始口服给药14天。在整个研究期间，所有小鼠在临床上保持正常。在开始抗生素治疗后21、60、90和120天，对小鼠标本进行组织学评价。组织学检查和特殊染色的结果表明，含阿莫西林的抗生素方案可预防进行性慢性肝炎和盲肠炎。在阿莫西林处理的小鼠的肝脏或盲肠中未观察到组织学上的螺旋菌。在未处理小鼠的肝脏和盲肠中以及用不含阿莫西林的抗生素方案处理的小鼠的盲肠中观察到螺旋菌。通过培养评估未处理小鼠和用阿莫西林处理的小鼠是否存在H。在开始抗生素治疗后60和90天通过聚合酶链反应检测肝细胞。所有未处理的小鼠粪便/盲肠培养试验呈阳性，五分之三的聚合酶链反应呈阳性。所有用阿莫西林治疗的小鼠对H.肝细胞培养和聚合酶链反应结果。通过饮水给scid幼鼠口服阿莫西林可预防H.肝
The use of antibiotic combinations to prevent acute and progressive chronic hepatitis and proliferative typhlitis associated with Helicobacter hepaticus infection in male scid/NCr mice was evaluated. The drug combinations used were amoxicillin-metronidazole-bismuth, tetracycline-metronidazole-bismuth, amoxicillin-neomycin, neomycin alone, and amoxicillin alone. Treatments were administered per os for 14 days beginning at 4 weeks of age. All mice remained clinically normal throughout the study. Specimens from mice were evaluated histologically at 21, 60, 90, and 120 days after initiation of the antibiotic treatments. Results of histologic examination and use of special stains indicated that the antibiotic regimens containing amoxicillin prevented progressive chronic hepatitis and typhlitis. Helical bacteria were not observed histologically in the liver or cecum of amoxicillin-treated mice. Helical bacteria were observed in the liver and cecum of untreated mice and in the cecum of mice treated with antibiotic regimens not containing amoxicillin. Untreated mice and those treated with amoxicillin were evaluated by culture for presence of H. hepaticus at 60 and 90 days and by polymerase chain reaction at 90 days after initiation of the antibiotic treatment. All untreated mice were test-positive by fecal/cecal culture, and three of five were positive by polymerase chain reaction. All mice treated with amoxicillin were negative for H. hepaticus by results of culture and polymerase chain reaction. The oral administration of amoxicillin to young scid mice via the drinking water prevents hepatitis and typhlitis caused by H. hepaticus.