Identification of the promoter sequences involved in the cell specific expression of the rat somatostatin gene.
Identification of the promoter sequences involved in the cell specific expression of the rat somatostatin gene.
复制标题
鉴定参与大鼠生长抑素基因的细胞特异性表达的启动子序列。
DOI：
10.1093/nar/15.14.5715
复制
发表时间：
1987
影响因子：
14.9
通讯作者：
Dixon,JE
中科院分区：
文献类型：
--
作者：
Andrisani,OM;Hayes,TE;Roos,B;Dixon,JE
含有大鼠生长抑素基因5‘侧翼区的DNA序列与细菌氯霉素乙酰转移酶基因的编码序列相连。该重组质粒可在神经源性生长抑素产生的CA-77细胞系中有效表达CAT活性。生长抑素启动子的缺失分析表明，该启动子的表达需要靠近-60位的序列。生长抑素启动子中-46到-43残基的4个碱基对的缺失导致了向下突变，提示在CA-77细胞中存在对启动子表达至关重要的元件。此外，生长抑素重组及其5‘端缺失构建体在CA-77细胞中优先表达CAT活性，而在HeLa、BSC4O和RIN-5F细胞中仅有基础水平的表达，这表明该启动子具有细胞特异性。
DNA sequences containing the 5′ flanking region of the rat somatoscatin gene were linked to the coding sequence of the bacterial chloramphenicol acetyl transferase gene. This recombinant plasmid is active in expressing CAT activity in the neuronally derived, somatostatin producing CA-77 cell line. Deletion analyses of the somatostatin promoter show that the sequences proximal to position -60, relative to the cap site are required for expression of this promoter. A 4 base pair deletion of residues -46 through -43 within the somatostatin promoter results in a down mutationin vivosuggesting the existence of an element critical for the expression of the promoter in CA-77 cells. In addition, the somatostatin recombinant and its 5′ deletion constructs preferentially express CAT activity in CA-77 cells, whereas only basal level of expression is observed in HeLa, BSC4O, and RIN-5F cell lines, pointing to the cell specific nature of this promoter.