Stimuli-Responsive Multilayers Based on Thiolated Polysaccharides That Affect Fibroblast Cell Adhesion.
Stimuli-Responsive Multilayers Based on Thiolated Polysaccharides That Affect Fibroblast Cell Adhesion.
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DOI：
10.1021/acsami.7b19022
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发表时间：
2018-02
影响因子：
9.5
通讯作者：
Pegah Esmaeilzadeh;Alexander Köwitsch;A. Liedmann;M. Menzel;B. Fuhrmann;Georg Schmidt;J. Klehm;T. Groth
中科院分区：
文献类型：
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作者：
Pegah Esmaeilzadeh;Alexander Köwitsch;A. Liedmann;M. Menzel;B. Fuhrmann;Georg Schmidt;J. Klehm;T. Groth
通过刺激响应聚合物平台控制生物材料的性能已成为近年来生物医学应用的一项重要技术。采用一层一层的涂覆策略，制备了巯基化壳聚糖（t-Chi）和巯基化硫酸软骨素（t-CS）的多层体系，该体系由5个双层（[t-Chi/t-CS]5）组成。为了代表一类新的化学可调纳米结构，通过在层形成过程中从pH 4上升到pH 9.3和使用氯胺- t （ChT）更强大的化学刺激来测试悬垂巯基交联的能力。经过这两种处理后，得到的多层膜表现出了依赖于刺激的行为，如它们的游离硫醇含量、润湿性、表面电荷、弹性模量、粗糙度、形貌、厚度和纤维连接蛋白的结合。与ph诱导的交联相比，人类真皮成纤维细胞的研究进一步表明，与ph诱导的交联相比，具有cht反应的多层膜作为细胞粘附表面具有良好的潜力。由于[t-Chi/t-CS]5多层系统对pH值和氧化还原环境等刺激有响应，因此具有二硫键形成的多层系统可能有助于调整它们与细胞的相互作用、膜降解和生物活性物质（如生长因子）的控制释放，以刺激响应的方式在未来的伤口愈合和组织工程应用中有用。
Control of the biomaterial properties through stimuli-responsive polymeric platforms has become an essential technique in recent biomedical applications. A multilayer system of thiolated chitosan (t-Chi) and thiolated chondroitin sulfate (t-CS), consisting of five double layers ([t-Chi/t-CS]5), was fabricated here by applying a layer-by-layer coating strategy. To represent a novel class of chemically tunable nanostructures, the ability to cross-link pendant thiol groups was tested by a rise from pH 4 during layer formation to pH 9.3 and a more powerful chemical stimulus by using chloramine-T (ChT). Following both treatments, the resulting multilayers showed stimuli-dependent behavior, as demonstrated by their content of free thiols, wettability, surface charge, elastic modulus, roughness, topography, thickness, and binding of fibronectin. Studies with human dermal fibroblasts further demonstrated the favorable potential of the ChT-responsive multilayers as a cell-adhesive surface compared to pH-induced cross-linking. Because the [t-Chi/t-CS]5 multilayer system is responsive to stimuli such as the pH and redox environment, multilayer systems with disulfide bond formation may help to tailor their interaction with cells, film degradation, and controlled release of bioactive substances like growth factors in a stimuli-responsive manner useful in future wound healing and tissue engineering applications.